DNA Methylation Regulated Nucleosome Dynamics

被引：55

作者：

Jimenez-Useche, Isabel ^{[1
]}

Ke, Jiaying ^{[1
]}

Tian, Yuqing ^{[1
]}

Shim, Daphne ^{[1
]}

Howell, Steven C. ^{[2
]}

Qiu, Xiangyun ^{[2
]}

Yuan, Chongli ^{[1
]}

机构：

[1] Purdue Univ, Sch Chem Engn, W Lafayette, IN 47907 USA

[2] George Washington Univ, Dept Phys, Washington, DC 20052 USA

来源：

SCIENTIFIC REPORTS | 2013年 / 3卷

关键词：

CPG-ISLANDS; CHROMATIN; CANCER; CONFORMATION; ORGANIZATION; COMPACTION; RESOLUTION; STABILITY; BINDING; TAILS;

D O I：

10.1038/srep02121

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A strong correlation between nucleosome positioning and DNA methylation patterns has been reported in literature. However, the mechanistic model accounting for the correlation remains elusive. In this study, we evaluated the effects of specific DNA methylation patterns on modulating nucleosome conformation and stability using FRET and SAXS. CpG dinucleotide repeats at 10 bp intervals were found to play different roles in nucleosome stability dependent on their methylation states and their relative nucleosomal locations. An additional (CpG)(5) stretch located in the nucleosomal central dyad does not alter the nucleosome conformation, but significant conformational differences were observed between the unmethylated and methylated nucleosomes. These findings suggest that the correlation between nucleosome positioning and DNA methylation patterns can arise from the variations in nucleosome stability dependent on their sequence and epigenetic content. This knowledge will help to reveal the detailed role of DNA methylation in regulating chromatin packaging and gene transcription.

引用

页数：5

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