Advanced oxidation protein products sensitized the transient receptor potential vanilloid 1 via NADPH oxidase 1 and 4 to cause mechanical hyperalgesia

被引:40
作者
Ding, Ruoting [1 ]
Jiang, Hui [1 ]
Sun, Baihui [2 ]
Wu, Xiaoliang [1 ]
Li, Wei [1 ]
Zhu, Siyuan [1 ]
Liao, Congrui [1 ]
Zhong, Zhaoming [1 ]
Chen, Jianting [1 ]
机构
[1] Southern Med Univ, Nanfang Hosp, Dept Spinal Surg, Guangzhou 510515, Guangdong, Peoples R China
[2] Southern Med Univ, Nanfang Hosp, Dept Plast & Aesthet Surg, Guangzhou 510515, Guangdong, Peoples R China
关键词
Advanced oxidation protein products; Hyperalgesia; Dorsal root ganglion; NADPH oxidase; TRPV1; DORSAL-ROOT GANGLION; ATTENUATES INFLAMMATORY PAIN; NEUROPATHIC PAIN; NEURONS; TRPV1; CONTRIBUTES; APOPTOSIS; STRESS; NOCICEPTION; ACTIVATION;
D O I
10.1016/j.redox.2016.09.004
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Oxidative stress is a possible pathogenesis of hyperalgesia. Advanced oxidation protein products (AOPPs), a new family of oxidized protein compounds, have been considered as a novel marker of oxidative stress. However, the role of AOPPs in the mechanism of hyperalgesia remains unknown. Our study aims to investigate whether AOPPs have an effect on hyperalgesia and the possible underlying mechanisms. To identify the AOPPs involved, we induced hyperalgesia in rats by injecting complete Freund's adjuvant (CFA) in hindpaw. The level of plasma AOPPs in CFA-induced rats was 1.6-fold in comparison with what in normal rats (P < 0.05). After intravenous injection of AOPPs-modified rat serum albumin (AOPPs-RSA) in Sprague-Dawley rats, the paw mechanical thresholds, measured by the electronic von Frey system, significantly declined. Immunofluorescence staining indicated that AOPPs increased expressions of NADPH oxidase 1 (Noxl), NADPH oxidase 4 (Nox4), transient receptor potential vanilloid 1 (TRPV1) and calcitonin gene-related peptide (CGRP) in the dorsal root ganglia (DRG) tissues. In-vitro studies were performed on primary DRG neurons which were obtained from both thoracic and lumbar DRG of rats. Results indicated that AOPPs triggered reactive oxygen species (ROS) production in DRG neurons, which were significantly abolished by ROS scavenger N-acetyl-f-cysteine (NAC) and small-interfering RNA (siRNA) silencing of Noxl or Nox4. The expressions of Noxl, Nox4, TRPV1 and CGRP were significantly increased in AOPPs-induced DRG neurons. And relevant siRNA or inhibitors notably suppressed the expressions of these proteins and the calcium influxes in AOPPs-induced DRG neurons. In conclusion, AOPPs increased significantly in CFA-induced hyperalgesia rats and they activated Noxl/Nox4-ROS to sensitize TRPV1-dependent Ca2+ influx and CGRP release which led to inducing mechanical hyperalgesia. (C) 2016 The Authors. Published by Elsevier B.V.
引用
收藏
页码:1 / 11
页数:11
相关论文
共 56 条
[1]   Protease-activated receptor 2 sensitizes the capsaicin receptor transient receptor potential vanilloid receptor 1 to induce hyperalgesia [J].
Amadesi, S ;
Nie, JJ ;
Vergnolle, N ;
Cottrell, GS ;
Grady, EF ;
Trevisani, M ;
Manni, C ;
Geppetti, P ;
McRoberts, JA ;
Ennes, H ;
Davis, B ;
Mayer, EA ;
Bunnett, NW .
JOURNAL OF NEUROSCIENCE, 2004, 24 (18) :4300-4312
[2]  
[Anonymous], DIABETES
[3]   CGRP receptors in the control of pain and inflammation [J].
Benemei, Silvia ;
Nicoletti, Paola ;
Capone, Jay G. ;
Geppetti, Pierangelo .
CURRENT OPINION IN PHARMACOLOGY, 2009, 9 (01) :9-14
[4]   Advanced oxidation protein products are generated by bovine neutrophils and inhibit free radical production in vitro [J].
Bordignon, Milena ;
Da Dalt, Laura ;
Marinelli, Lieta ;
Gabai, Gianfranco .
VETERINARY JOURNAL, 2014, 199 (01) :162-168
[5]   Age-Related Oxidative Stress Compromises Endosomal Proteostasis [J].
Cannizzo, Elvira S. ;
Clement, Cristina C. ;
Morozova, Kateryna ;
Valdor, Rut ;
Kaushik, Susmita ;
Almeida, Larissa N. ;
Follo, Carlo ;
Sahu, Ranjit ;
Cuervo, Ana Maria ;
Macian, Fernando ;
Santambrogio, Laura .
CELL REPORTS, 2012, 2 (01) :136-149
[6]   Localization of NADPH oxidase in sympathetic and sensory ganglion neurons and perivascular nerve fibers [J].
Cao, Xian ;
Demel, Stacie L. ;
Quinn, Mark T. ;
Galligan, James J. ;
Kreulen, David .
AUTONOMIC NEUROSCIENCE-BASIC & CLINICAL, 2009, 151 (02) :90-97
[7]   Spinal sigma-1 receptors activate NADPH oxidase 2 leading to the induction of pain hypersensitivity in mice and mechanical allodynia in neuropathic rats [J].
Choi, Sheu-Ran ;
Roh, Dae-Hyun ;
Yoon, Seo-Yeon ;
Kang, Suk-Yun ;
Moon, Ji-Young ;
Kwon, Soon-Gu ;
Choi, Hoon-Seong ;
Han, Ho-Jae ;
Beitz, Alvin J. ;
Oh, Seog-Bae ;
Lee, Jang-Hern .
PHARMACOLOGICAL RESEARCH, 2013, 74 :56-67
[8]   RETRACTED: Rutaecarpine inhibits hypoxia/reoxygenation-induced apoptosis in rat hippocampal neurons (Retracted Article) [J].
Dai, Zhong ;
Xiao, Jian ;
Liu, Si-Yu ;
Cui, Liao ;
Hu, Gao-Yun ;
Jiang, De-Jian .
NEUROPHARMACOLOGY, 2008, 55 (08) :1307-1312
[9]   Iron therapy, advanced oxidation protein products, and carotid artery intima-media thickness in end-stage renal disease [J].
Drüeke, T ;
Witko-Sarsat, V ;
Massy, Z ;
Descamps-Latscha, B ;
Guerin, AP ;
Marchais, SJ ;
Gausson, V ;
London, GM .
CIRCULATION, 2002, 106 (17) :2212-2217
[10]   Persistent Nociception Triggered by Nerve Growth Factor (NGF) Is Mediated by TRPV1 and Oxidative Mechanisms [J].
Eskander, Michael A. ;
Ruparel, Shivani ;
Green, Dustin P. ;
Chen, Paul B. ;
Por, Elaine D. ;
Jeske, Nathaniel A. ;
Gao, Xiaoli ;
Flores, Eric R. ;
Hargreaves, Kenneth M. .
JOURNAL OF NEUROSCIENCE, 2015, 35 (22) :8593-8603