Presence of three distinct genotypes within the Paragonimus westermani complex in northeastern India

被引：20

作者：

Devi, K. Rekha ^{[1
,2
]}

Narain, Kanwar ^{[2
]}

Mahanta, Jagadish ^{[2
]}

Nirmolia, Tulika ^{[2
]}

Blair, David ^{[3
]}

Saikia, Sidhartha P. ^{[2
]}

Agatsuma, Takeshi ^{[1
]}

机构：

[1] Kochi Med Sch, Dept Environm Hlth Sci, Nankoku, Kochi 7838505, Japan

[2] NE Reg Indian Council Med Res, Reg Med Res Ctr, Dibrugarh 786001, Assam, India

[3] James Cook Univ, Sch Marine & Trop Biol, Townsville, Qld 4811, Australia

来源：

PARASITOLOGY | 2013年 / 140卷 / 01期

关键词：

Paragonimus westermani complex; lung flukes; India; MOLECULAR PHYLOGENETIC STATUS; LUNG FLUKE; IDENTIFICATION; HETEROTREMUS; DISCOVERY; INFECTION; VIETNAM; AREA;

D O I：

10.1017/S0031182012001229

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The name Paragonimus westermani (Kerbert, 1878) is commonly applied to members of a species complex that includes the well-known Asian lung fluke of medical and veterinary importance. Unambiguous molecular and morphological evidence showing the presence of a member of the complex in India has recently been published. In the present study we report the occurrence of 2 more members of the P. westermani complex in northeastern (NE) India. Surveys of the freshwater crabs Maydelliatelphusa lugubris in NE India revealed 2 morphologically distinct types of lung fluke metacercariae. Phylogenetic analyses, using DNA sequences from ITS2, 28S and cox1 gene regions indicate that these lung metacercariae belong to P. westermani complex. Type 1 metacercariae have a more basal position within the complex whereas type 2 metacercariae are closely related to the relatively derived forms of P. westermani from NE Asia (Japan, Korea, China) and Vietnam. A third type of metacercaria (type 3), detected in another crab host, Sartoriana spinigera in Assam, was phylogenetically close to P. siamensis, also a member of the P. westermani group. Molecular evidence has demonstrated the existence of 3 genotypes of lung flukes within the Paragonimus westermani complex in NE India. Two of these were previously unknown.

引用

页码：76 / 86

页数：11

共 34 条

[21]

MIYAZAKI I, 1981, Medical Bulletin of Fukuoka University, V8, P153

[22]

Miyazaki I., 1965, Japanese Journal of Parasitology, V14, P251

[23] Discovery of Paragonimus proliferus in Northern Vietnam and their molecular phylogenetic status among genus Paragonimus [J].

Pham Ngoc Doanh ;

Shinohara, Akio ;

Horii, Yoichiro ;

Habe, Shigehisa ;

Nawa, Yukifumi ;

Nguyen Thi Le .

PARASITOLOGY RESEARCH, 2008, 102 (04) :677-683

[24] Human paragonimiasis in Viet Nam: Epidemiological survey and identification of the responsible species by DNA sequencing of eggs in patients' sputum [J].

Pham Ngoc Doanh ;

Do Trung Dung ;

Dang Thi Cam Thach ;

Horii, Yoichiro ;

Shinohara, Akio ;

Nawa, Yukifumi .

PARASITOLOGY INTERNATIONAL, 2011, 60 (04) :534-537

[25] Selecting the best-fit model of nucleotide substitution [J].

Posada, D ;

Crandall, KA .

SYSTEMATIC BIOLOGY, 2001, 50 (04) :580-601

[26] Paragonimus heterotremus infection in Nagaland: A new focus of paragonimiasis in India [J].

Singh, T. S. ;

Sugiyama, H. ;

Umehara, A. ;

Hiese, S. ;

Khalo, K. .

INDIAN JOURNAL OF MEDICAL MICROBIOLOGY, 2009, 27 (02) :123-127

[27] Food-Borne Trematodiases in Southeast Asia: Epidemiology, Pathology, Clinical Manifestation and Control [J].

Sripa, Banchob ;

Kaewkes, Sasithorn ;

Intapan, Pewpan M. ;

Maleewong, Wanchai ;

Brindley, Paul J. .

ADVANCES IN PARASITOLOGY, VOL 72: IMPORTANT HELMINTH INFECTIONS IN SOUTHEAST ASIA: DIVERSITY AND POTENTIAL FOR CONTROL AND ELIMINATION, PT A, 2010, 72 :305-350

[28]

SUGIYAMA H, 1983, JPN J VET SCI, V45, P227

[29]

Sugiyama H., 2007, SE ASIAN J TROPICAL, V38, P87

[30] MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods [J].

Tamura, Koichiro ;

Peterson, Daniel ;

Peterson, Nicholas ;

Stecher, Glen ;

Nei, Masatoshi ;

Kumar, Sudhir .

MOLECULAR BIOLOGY AND EVOLUTION, 2011, 28 (10) :2731-2739

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