Metabolism Links Bacterial Biofilms and Colon Carcinogenesis

被引：292

作者：

Johnson, Caroline H. ^{[1
]}

Dejea, Christine M. ^{[2
]}

Edler, David ^{[3
]}

Hoang, Linh T. ^{[1
]}

Santidrian, Antonio F. ^{[4
,5
]}

Felding, Brunhilde H. ^{[4
,5
]}

Ivanisevic, Julijana ^{[1
]}

Cho, Kevin ^{[6
,7
,8
]}

Wick, Elizabeth C. ^{[9
]}

Hechenbleikner, Elizabeth M. ^{[9
]}

Uritboonthai, Winnie ^{[1
]}

Goetz, Laura ^{[10
]}

Casero, Robert A., Jr. ^{[11
,12
,13
]}

Pardoll, Drew M. ^{[11
,12
,13
]}

White, James R. ^{[14
]}

Patti, Gary J. ^{[6
,7
,8
]}

Sears, Cynthia L. ^{[2
,11
,12
,13
]}

Siuzdak, Gary ^{[1
]}

机构：

[1] Scripps Res Inst, Scripps Ctr Metabol & Mass Spectrometry, La Jolla, CA 92037 USA

[2] Johns Hopkins Med Inst, Bloomberg Sch Publ Hlth, Dept Mol Microbiol & Immunol, Baltimore, MD 21218 USA

[3] Karolinska Univ Hosp, Ctr Surg Gastroenterol, S-17177 Stockholm, Sweden

[4] Scripps Res Inst, Dept Physiol Chem, La Jolla, CA 92037 USA

[5] Scripps Res Inst, Dept Mol & Expt Med, La Jolla, CA 92037 USA

[6] Washington Univ, Sch Med, Dept Chem, St Louis, MO 63110 USA

[7] Washington Univ, Sch Med, Dept Genet, St Louis, MO 63110 USA

[8] Washington Univ, Sch Med, Dept Med, St Louis, MO 63110 USA

[9] Johns Hopkins Univ, Sch Med, Johns Hopkins Med Inst, Dept Surg, Baltimore, MD 21218 USA

[10] Scripps Clin Med Grp, Dept Surg, La Jolla, CA 92037 USA

[11] Johns Hopkins Med Inst, Johns Hopkins Sch Med, Dept Oncol, Baltimore, MD 21218 USA

[12] Johns Hopkins Med Inst, Johns Hopkins Sch Med, Dept Med, Baltimore, MD 21218 USA

[13] Johns Hopkins Med Inst, Johns Hopkins Sch Med, Sidney Kimmel Comprehens Canc Ctr, Baltimore, MD 21218 USA

[14] Resphera Biosci, Baltimore, MD 21231 USA

来源：

CELL METABOLISM | 2015年 / 21卷 / 06期

基金：

美国国家卫生研究院;

关键词：

COLORECTAL-CANCER; MASS-SPECTROMETRY; MUCOSAL FLORA; POLYAMINES; ORGANIZATION; MICROBIOTA; DISEASE;

D O I：

10.1016/j.cmet.2015.04.011

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Bacterial biofilms in the colon alter the host tissue microenvironment. A role for biofilms in colon cancer metabolism has been suggested but to date has not been evaluated. Using metabolomics, we investigated the metabolic influence that microbial biofilms have on colon tissues and the related occurrence of cancer. Patient-matched colon cancers and histologically normal tissues, with or without biofilms, were examined. We show the upregulation of polyamine metabolites in tissues from cancer hosts with significant enhancement of N-1, N-12-diacetylspermine in both biofilm-positive cancer and normal tissues. Antibiotic treatment, which cleared biofilms, decreased N-1, N-12 -diacetylspermine levels to those seen in biofilm-negative tissues, indicating that host cancer and bacterial biofilm structures contribute to the polyamine metabolite pool. These results show that colonic mucosal biofilms alter the cancer metabolome to produce a regulator of cellular proliferation and colon cancer growth potentially affecting cancer development and progression.

引用

页码：891 / 897

页数：7

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