Expression, purification, crystallization and preliminary X-ray crystallographic analysis of Zucchini from Drosophila melanogaster

被引：1

作者：

Fukuhara, Satoshi ^{[1
]}

Nishimasu, Hiroshi ^{[1
]}

Bonnefond, Luc ^{[1
]}

Matsumoto, Naoki ^{[1
]}

Ishitani, Ryuichiro ^{[1
]}

Nureki, Osamu ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Sci, Dept Biophys & Biochem, Tokyo 1130032, Japan

来源：

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2012年 / 68卷

基金：

日本学术振兴会;

关键词：

PRIMARY PIRNA BIOGENESIS; NUAGE FORMATION; SMALL RNAS; GERMLINE; MITOPLD; PATHWAY; GENOME; FAMILY; PIWI;

D O I：

10.1107/S1744309112038936

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

PIWI-interacting RNAs (piRNAs) bind PIWI proteins and silence transposons to maintain the genomic integrity of germ cells. Zucchini (Zuc), a phospholipase D superfamily member, is conserved among animals and is implicated in piRNA biogenesis. However, the underlying mechanism by which Zuc participates in piRNA biogenesis remains elusive. Drosophila melanogaster Zuc (DmZuc) was expressed in Escherichia coli, purified and crystallized. X-ray diffraction data were collected to 1.75 angstrom resolution. The crystal belonged to space group P2(1), with unit-cell parameters a = 55.0, b = 71.2, c = 56.3 A, beta = 107.9 degrees.

引用

页码：1346 / 1350

页数：5

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