Effect of Bacteriophage in Enterotoxigenic Escherichia coli (ETEC) Infected Pigs

被引:36
作者
Cha, Seung Bin
Yoo, An Na
Lee, Won Jung
Shin, Min Kyoung
Jung, Myung Hwan
Shin, Seun Won
Cho, Young Wook [2 ]
Yoo, Han Sang [1 ]
机构
[1] Seoul Natl Univ, Coll Vet Med, KRF Zoonot Dis Prior Res Inst, Dept Infect Dis,Kwanak Ku, Seoul 151742, South Korea
[2] CJ CheilJedang Co, CJ Res Inst Biotechnol, Seoul 157724, South Korea
关键词
bacteriophage; ETEC; prophylactic efficacy; swine; DIARRHEA; THERAPY;
D O I
10.1292/jvms.11-0556
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
We evaluated effect of enterotoxigenic Escherichia coli (ETEC) specific lytic phage 012 in ETEC infected pigs. Phage was mixed with feed at a ratio of 1:1,000 (0.1%). One week after initially providing phage mixed feed, pigs were challenged orally with 10(11) CFU of ETEC and body weight, diarrhea score, bacterial CFU and phage PFU in the feces were measured. Pigs of phage treated groups C (10(6) PFU/g) and D (10(8) PFU/g) showed more resistance to diarrhea due to ETEC infection compared to positive control group B on the third day after the initial challenge. Moreover, during the quantitation of ETEC in feces, both groups C and D showed approximately 63.92 and 60.73% reduced ETEC compared to positive control group B. Phages were successfully isolated from feces in both groups C and D during the experiment without any adverse effects, suggesting the possibility of using CJ12 as a feed additive.
引用
收藏
页码:1037 / 1039
页数:3
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