Photoelectrochemical biosensor for acetylcholinesterase activity study based on metal oxide semiconductor nanocomposites

被引：9

作者：

Huang, Qilin ^{[1
,2
]}

Wang, Ya ^{[1
]}

Lei, Li ^{[1
]}

Xu, Zhiai ^{[1
]}

Zhang, Wen ^{[1
]}

机构：

[1] East China Normal Univ, Sch Chem & Mol Engn, Shanghai 200241, Peoples R China

[2] Yuxi Normal Univ, Dept Chem, Yuxi 653100, Peoples R China

来源：

JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 2016年 / 781卷

基金：

中国国家自然科学基金;

关键词：

Photoelectrochemical biosensor; TiO2; nanotubes; Nanocomposite; Acetylcholinesterase; Cadmium ion; PHOTOCATALYTIC ACTIVITY; ANTIOXIDANT STATUS; AU NANOPARTICLES; IN-VITRO; CADMIUM; LIGHT; INHIBITION; NANOTUBES; RATS; GROWTH;

D O I：

10.1016/j.jelechem.2016.07.007

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Acetylcholinesterase (AChE) is a well-known serine hydrolase and its dysfunction could disturb cholinergic neurotransmission which is related to the pathogenesis of neurodegenerative disorders. In this report, a photoelectrochemical biosensor based on metal oxide semiconductor nanocomposite was fabricated to investigate the effect of cadmium ion (Cd2+) on the activity of AChE. The photoelectrochemical nanocomposite was prepared by anodic oxidation of titanium (Ti) foil to form titanium dioxide (TiO2) nanotubes (TNs) array followed by cathodic deposition of zinc oxide nanorods (ZnONRs) onto the TNs. AChE was immobilized on the obtained nanocomposites and the biosensor showed enhanced photoelectrochemical response under visible light irradiation. The experimental results showed that Cd2+ exhibited interesting dose-dependent and time-dependent effects on AChE activity. Specifically, high concentration of Cd2+ inhibited while low level of Cd2+ could stimulate the activity of AChE. These findings are of great significance for the study of enzyme activity influenced by metal ions and related pathogenesis investigation of neurodegenerative disorders. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：377 / 382

页数：6

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