Premature senescence in human breast cancer and colon cancer cells by tamoxifen-mediated reactive oxygen species generation

Aims: Cellular senescence is an important tumor suppression process in vivo. Tamoxifen is a well-known anti-breast cancer drug; however, its molecular function is poorly understood. Here, we examined whether tamoxifen promotes senescence in breast cancer and colon cancer cells for the first time. Main methods: Human breast cancer MCF-7, T47D, and MDA-MB-435 and colorectal cancer HCT116 cells were treated with tamoxifen. Cellular senescence was measured by SA-S-gal staining and based on the protein expression of p53 and p21(C1p1/WAF1). The production of reactive oxygen species (ROS) was determined by staining with CM-H(2)DCFDA and dihydroethidium (DHE). CK2 activity was assessed with a specific peptide substrate. Key findings: Tamoxifen promoted senescence phenotype and ROS generation in MCF-7 and HCT116 cells. The ROS scavenger, N-acetyl-L-cysteine (NAC), and the NADPH oxidase inhibitor, apocynin, almost completely abolished this event. Tamoxifen inhibited the catalytic activity of CK2. Overexpression of CK2 alpha antagonized senescence mediated by tamoxifen, indicating that tamoxifen induced senescence via a CK2-dependent pathway. A well-known CK2 inhibitor, 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB), also stimulated ROS production and senescence in MCF-7 cells. Finally, experiments using T47D (wild-type p53) and MDA-MB-435 (mutant p53) cell lines suggested that tamoxifen induces p53-independent ROS production as well as p53-dependent senescence in breast cancer cells. Significance: These results demonstrate that tamoxifen promotes senescence through a ROS-p53-p21(Cipl/WAF1) dependent pathway by inhibiting CK2 activity in breast cancer and colon cancer cells. (C) 2013 Elsevier Inc. All rights reserved.