Label-free determination of protein-surface interaction kinetics by ionic conductance inside a nanochannel

被引：34

作者：

Durand, Nicolas F. Y. ^{[1
]}

Renaud, Philippe ^{[1
]}

机构：

[1] Ecole Polytech Fed Lausanne, STI LMIS, Microsyst Lab, CH-1015 Lausanne, Switzerland

来源：

LAB ON A CHIP | 2009年 / 9卷 / 02期

关键词：

HYDROPHOBIC SURFACES; COMPETITIVE BEHAVIOR; RELAXATION KINETICS; HISTORY DEPENDENCE; ADSORPTION; ALBUMIN; FIBRINOGEN; SALT; FLOW; TIRF;

D O I：

10.1039/b811006a

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a label-free conductometric platform for the rapid measurement of kinetics parameters for the adsorption and desorption of proteins on surfaces. Adsorbed Bovine Serum Albumin (BSA) has been detected electrically with response times in the minute range, and kinetic models were elaborated and compared to the measurements. The device presents similar characteristics to Surface Plasmon Resonance (SPR) immunosensors, but takes advantage of a simpler, low-cost electronic measurement unit.

引用

页码：319 / 324

页数：6

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