The Frequency Response and Optical Detection Characteristics of a Series Photodetector Frequency Circuit System Matched With Polydimethylsiloxance (PDMS)

被引：0

作者：

Hsu, Y. M. ^{[1
,2
]}

Chang, C. C. ^{[1
]}

机构：

[1] Natl Taiwan Ocean Univ, Dept Elect Engn, Keelung 20224, Taiwan

[2] Lan Yang Inst Technol, Dept Innovat Digital Living, Ilan 26141, Taiwan

来源：

LASERS IN ENGINEERING | 2013年 / 25卷 / 3-4期

关键词：

Series photodetector frequency circuit system; laser; polydimethylsiloxance (PDMS); fluorescence dye concentration; microfluidic chip; DNA HYBRIDIZATION;

D O I：

暂无

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

This study elucidates the frequency response and optical detection characteristics of series photodetector frequency circuit system matched with polydimethylsiloxance (PDMS) for detection of fluorescence dye concentration. While the same fluorescence intensity irradiated on the photodetector, the series photodetector frequency circuit system with higher responsivity of photodetector had higher frequency sensitivity. In accordance with Cy5 fluorescence dye concentrations and frequency responses, the detection limit of fluorescence dye concentration 2 pmol/l can be measured by 100 MHz sensor system matched with PDMS. The results also showed that the frequency shift of the sensor system matched with photodetector PT was linearly related to the logarithm of fluorescence dye concentration from 2 to 20 mu mol/l. Moreover, the frequency sensor system had a potential application in the detection of deoxyribonucleic acid (DNA) hybridization with PDMS microfluidic chip.

引用

页码：133 / 149

页数：17

共 15 条

[1] A monolithic silicon based integrated signal generation and detection system for monitoring DNA hybridisation [J].

Bertolino, C ;

MacSweeney, M ;

Tobin, J ;

O'Neill, B ;

Sheehan, MM ;

Coluccia, S ;

Berney, H .

BIOSENSORS & BIOELECTRONICS, 2005, 21 (04) :565-573

[2] Characterization of DNA immobilization and subsequent hybridization using in situ quartz crystal microbalance, fluorescence spectroscopy, and surface plasmon resonance [J].

Cho, YK ;

Kim, S ;

Kim, YA ;

Lim, HK ;

Lee, K ;

Yoon, DS ;

Lim, G ;

Pak, YE ;

Ha, TH ;

Kim, K .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2004, 278 (01) :44-52

[3] Electrochemical investigation of DNA-modified surfaces: From quantitation methods to experimental conditions [J].

Ge, Bixia ;

Huang, Yu-Chuan ;

Sen, Dipankar ;

Yu, Hua-Zhong .

JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 2007, 602 (02) :156-162

[4] Frequency dependent and surface characterization of DNA immobilization and hybridization [J].

Hang, TC ;

Guiseppi-Elie, A .

BIOSENSORS & BIOELECTRONICS, 2004, 19 (11) :1537-1548

[5] A novel frequency method for quantitative analysis of fluorescence dye concentration by using series photodetector frequency circuit system [J].

Hsu, Yuh Ming ;

Chang, Chung Cheng .

SENSORS AND ACTUATORS A-PHYSICAL, 2009, 154 (01) :23-29

[6]

Kasap S.O., 2003, HDB OPTOELECTRONICS

[7] Advantages of peptide nucleic acid oligonucleotides for sensitive site directed 16S rRNA fluorescence in situ hybridization (FISH) detection of Campylobacter jejuni, Campylobacter coli and Campylobacter lari [J].

Lehtola, MJ ;

Loades, CJ ;

Keevil, CW .

JOURNAL OF MICROBIOLOGICAL METHODS, 2005, 62 (02) :211-219

[8] DNA hybridization assays using metal-enhanced fluorescence [J].

Malicka, J ;

Gryczynski, I ;

Lakowicz, JR .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2003, 306 (01) :213-218

[9] Detection of a K-ras point mutation employing peptide nucleic acid at the surface of a SPR biosensor [J].

Sato, Y ;

Fujimoto, K ;

Kawaguchi, H .

COLLOIDS AND SURFACES B-BIOINTERFACES, 2003, 27 (01) :23-31

[10]

Setian L, 2002, HDB APPL ELECTROOPTI

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