Fluorescence-Labeled Immunomicelles: Preparation, in vivo Biodistribution, and Ability to Cross the Blood-Brain Barrier

被引：12
作者：
Yue, Jun [1 ,2 ]
Liu, Shi [1 ]
Wang, Rui [1 ,2 ]
Hu, Xiuli [1 ]
Xie, Zhigang [1 ]
Huang, Yubin [1 ]
Jing, Xiabin [1 ]
机构：
[1] Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Polymer Phys & Chem, Changchun 130022, Peoples R China
[2] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
来源：
MACROMOLECULAR BIOSCIENCE | 2012年 / 12卷 / 09期
基金：
中国国家自然科学基金;
关键词：
biodegradable; biodistribution; blood-brain barrier; micelles; OX26; AMPHIPHILIC BLOCK-COPOLYMERS; DRUG-DELIVERY; TRANSFERRIN RECEPTOR; MONOCLONAL-ANTIBODY; GENE-EXPRESSION; P-GLYCOPROTEIN; CANCER-THERAPY; NANOPARTICLES; TRANSPORTERS; TUMORS;
D O I：
10.1002/mabi.201200037
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Multifunctional hybrid micelles are prepared from amphiphilic mal-PEG-b-PLA and mPEG-b-P(LA-co-DHC/RhB) block copolymers. A specific anti-transferrin receptor antibody, OX26, is linked onto the surface of the micelles. ELISA indicates that the conjugated antibody preserves its activity. OX26 conjugation can increase the uptake efficiency of micelles by target cell lines (C6). Pharmacokinetics and in vivo biodistribution experiments are carried out to investigate the ability of OX26-conjugated micelles (immunomicelles) to cross the bloodbrain barrier. The data show that the brain uptake of OX26-conjugated micelles is much more than that of OX26-free ones. Therefore, OX26-conjugated micelles will be promising drug carriers to cross the blood-brain barrier.
引用
收藏
页码：1209 / 1219
页数：11
相关论文
共 50 条
[1]   Mechanisms of phagocytosis in macrophages [J].
Aderem, A ;
Underhill, DM .
ANNUAL REVIEW OF IMMUNOLOGY, 1999, 17 :593-623
[2]   Development and brain delivery of chitosan-PEG nanoparticles functionalized with the monoclonal antibody OX26 [J].
Aktas, Y ;
Yemisci, M ;
Andrieux, K ;
Gürsoy, RN ;
Alonso, MJ ;
Fernandez-Megia, E ;
Novoa-Carballal, R ;
Quiñoá, E ;
Riguera, R ;
Sargon, MF ;
Çelik, HH ;
Demir, AS ;
Hincal, AA ;
Dalkara, T ;
Çapan, Y ;
Couvreur, P .
BIOCONJUGATE CHEMISTRY, 2005, 16 (06) :1503-1511
[3]   Active targeting of brain tumors using nanocarriers [J].
Beduneau, Arnaud ;
Saulnier, Patrick ;
Benoit, Jean-Pierre .
BIOMATERIALS, 2007, 28 (33) :4947-4967
[4]   Design of targeted lipid nanocapsules by conjugation of whole antibodies and antibody Fab' fragments [J].
Beduneau, Arnaud ;
Saulnier, Patrick ;
Hindre, Francois ;
Clavreul, Anne ;
Leroux, Jean-Christophe ;
Benoit, Jean-Pierre .
BIOMATERIALS, 2007, 28 (33) :4978-4990
[5]   HETEROBIFUNCTIONAL POLY(ETHYLENE OXIDE) - SYNTHESIS OF ALPHA-METHOXY-OMEGA-AMINO AND ALPHA-HYDROXY-OMEGA-AMINO PEOS WITH THE SAME MOLECULAR-WEIGHTS [J].
CAMMAS, S ;
NAGASAKI, Y ;
KATAOKA, K .
BIOCONJUGATE CHEMISTRY, 1995, 6 (02) :226-230
[6]   Self-Assembly Strategy for the Preparation of Polymer-Based Nanoparticles for Drug and Gene Delivery [J].
Chen, Si ;
Cheng, Si-Xue ;
Zhuo, Ren-Xi .
MACROMOLECULAR BIOSCIENCE, 2011, 11 (05) :576-589
[7]   Intra-arterial carboplatin chemotherapy for brain tumors: A dose escalation study based on cerebral blood flow [J].
Cloughesy, TF ;
Gobin, YP ;
Black, KL ;
Vinuela, F ;
Taft, F ;
Kadkhoda, B ;
Kabbinavar, F .
JOURNAL OF NEURO-ONCOLOGY, 1997, 35 (02) :121-131
[8]   Nanoparticle therapeutics: an emerging treatment modality for cancer [J].
Davis, Mark E. ;
Chen, Zhuo ;
Shin, Dong M. .
NATURE REVIEWS DRUG DISCOVERY, 2008, 7 (09) :771-782
[9]   The blood-brain barrier and cancer:: Transporters, treatment, and Trojan horses [J].
Deeken, John F. ;
Loescher, Wolfgang .
CLINICAL CANCER RESEARCH, 2007, 13 (06) :1663-1674
[10]   Drug transport to the brain:: Key roles for the efflux pump P-glycoprotein in the blood-brain barrier [J].
Demeule, M ;
Régina, A ;
Jodoin, J ;
Laplante, A ;
Dagenais, C ;
Berthelet, F ;
Moghrabi, A ;
Béliveau, R .
VASCULAR PHARMACOLOGY, 2002, 38 (06) :339-348
12345