INFLUENCE OF COENZYME Q10 AND QUERCETIN IN ANTIOXIDANT ENZYME ACTIVITY IN MOUSE BRAIN INFLAMMATION

During the inflammatory process, oxygen free radicals are produced, causing a balance in the oxidative-reducing reactions that occur in every cell of the body. In order to counter ROS, many mechanisms have been developed in the body to protect against the accumulation of harmful forms of oxygen and to participate in their transformation into inactive derivatives. These mechanisms include compounds of exogenous and endogenous origin that form an antioxidant system with enzymatic properties (SOD, GSHPx, CAT) and non-enzymatic (GSH, coenzyme Q10, quercetin). In the case of significant growth of oxygen radicals and generation, in the absence of an effective antioxidant system, defense mechanisms may be reduced, and consequently, many diseases (including neurodegenerative diseases, heart disease, cancer) may develop. Therefore, it is extremely important to look for compounds that support enzymatic defense mechanisms of the body. The present study was designed to evaluate the possible protective effect of coenzyme Q10 or quercetin, for the treatment of zymosan induced inflammation in the mouse brain. The obtained results were subjected to multivariate analysis of variance MANOVA. Post -hoc analysis was performed using the Tukey test. Differences were considered statistically significant at p<0.05. The analysis demonstrated that the use of zymosan induces oxidative stress manifested by a decrease in SOD and GPx acivity and GSH concentrations. The results of the statistical analysis showed that coenzyme Q and quercetin had a significant effect on antioxidant concentration in the mouse brain, also the duration of coenzyme Q and quercetin was found to be significant. Conducted studies have shown that coenzyme Q10 and quercetin increase SOD, GSHPx, CAT activities and GSH levels in the brain, thus eliminating oxidative stress. In conclusion, a balanced diet supplemented with coenzyme Q10 and quercetin can increase the efficiency of antioxidant enzymes in the brain, helping to eliminate pathological conditions.