Role of Nitric Oxide in the Pathogenesis of Alloxan Diabetes

被引：6

作者：

Belkina, L. M. ^{[1
]}

Smirnova, E. A. ^{[1
]}

Terekhina, O. L. ^{[1
]}

Kruglov, S. V. ^{[1
]}

Boichuk, E. S. ^{[1
]}

机构：

[1] Russian Acad Med Sci, Inst Pathol & Pathophysiol, Moscow, Russia

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2013年 / 154卷 / 05期

关键词：

alloxan diabetes; August and Wistar rats; L-NNA; nitric oxide; NF-KAPPA-B; OXIDATIVE STRESS; L-ARGININE; BETA-CELLS; RATS; MELLITUS;

D O I：

10.1007/s10517-013-2009-4

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

We studied the effects of N-omega-nitro-L-arginine (L-NNA), a nonselective inhibitor of NO synthases, on the severity of type 1 diabetes mellitus induced by subcutaneous injection of 130 mg/kg alloxan in August rats with high activity of NO system and in Wistar rats. Five days after alloxan injection, hyperglycemia levels after overnight fasting in August and Wistar rats were 27.1 +/- 3.7 and 22.0 +/- 1.1 mmol/liter, respectively (p < 0.03). The mortality over 15 days after alloxan injection in August rats was higher than in Wistar rats (36 and 26%, respectively). L-NNA normalized glucose levels in diabetics of both groups. It completely prevented mortality in August and reduced it to 13% in Wistar rats. Body weight loss and polydipsia after LNNA injection were also less pronounced in August rats. Plasma nitrite/nitrate concentrations in August rats were 32% higher than in Wistar rats, both in intact and diabetic rats. These data attest to an important role of NO in the pathogenesis of alloxan diabetes.

引用

页码：602 / 605

页数：4

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