Follicular growth after xenotransplantation of cryopreserved/thawed human ovarian tissue in SCID mice: dynamics and molecular aspects

被引:34
作者
Ayuandari, Sarrah [1 ,2 ]
Winkler-Crepaz, Katharina [1 ]
Paulitsch, Monika [1 ,3 ]
Wagner, Cora [1 ]
Zavadil, Claudia [4 ]
Manzl, Claudia [4 ]
Ziehr, Stephanie C. [1 ,5 ]
Wildt, Ludwig [1 ]
Hofer-Tollinger, Susanne [1 ]
机构
[1] Med Univ Innsbruck, Dept Gynecol Endocrinol & Reprod Med, Anichstr 35, A-6020 Innsbruck, Austria
[2] Gadjah Mada Univ, Dept Obstet & Gynecol, Fac Med, Yogyakarta 55281, Indonesia
[3] Univ Innsbruck, Inst Zool, Technikerstr 25, A-6020 Innsbruck, Austria
[4] Med Univ Innsbruck, Dept Pathol, Mullerstr 44, A-6020 Innsbruck, Austria
[5] ART Bogenhausen, Prinzregentenstr 69, D-81675 Munich, Germany
关键词
Fertility preservation; Human ovarian tissue xenotransplantation; Human ovarian tissue cryopreservation; Follicular recruitment; PTEN; OOCYTE-SPECIFIC DELETION; FERTILITY PRESERVATION; PRIMORDIAL FOLLICLES; TRANSPLANTATION; ACTIVATION; CANCER; VITRIFICATION; STIMULATION; WOMEN; PTEN;
D O I
10.1007/s10815-016-0769-2
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
To study the influence of xenotransplantation on follicular recruitment and growth in cryopreserved/thawed human ovarian tissue. Two 3-mm pieces of cryopreserved/thawed human ovarian tissue obtained from female cancer patients (n = 11) were xenotransplanted into a subcutaneous neck pouch of 6-week-old ovarectomized SCID mice (n = 33) for 4 (n = 18) and 12 (n = 15) weeks. Thirty-two out of 33 mice survived the entire observation periods. Graft recovery rate was 95.58 % (65 of 68 grafts). The percentages of primordial follicles after 4 weeks (P < 0.001) and 12 weeks (P = 0.009) of grafting were significantly lower in comparison to pregraft controls. The percentage of secondary follicle was significantly higher after 4 weeks of grafting (P = 0.018) and after 12 weeks (P = 0.001) of grafting in comparison to pregraft controls. Ki67 immunohistochemistry showed that proliferative follicles were significantly higher after 4 and 12 weeks of grafting compared to pregraft controls (P < 0.001). All follicles analyzed by TUNEL staining appeared healthy after xenotransplantation. The expression level of PTEN was reduced by 2.47-fold after 4 weeks of xenotransplantation, and this result was significant when 2(-Delta Ct) were analyzed (P = 0.042). The higher proportion of growing follicles compared to resting follicles observed after xenotransplantation is most likely due to downregulation of PTEN gene expression followed by acceleration of follicular recruitment.
引用
收藏
页码:1585 / 1593
页数:9
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