A new small molecule inhibits Streptococcus mutans biofilms invitro and in vivo

AimsThe aim of this study was to identify new small molecules that can inhibit Streptococcus mutans biofilms by invitro and invivo model. Methods and ResultsWe evaluated the effect of a small molecule 2-amino-imidazole/triazole conjugate (2-AI/T) on the formation of Strep.mutans biofilms by culturing in 96-well plates. Toxicity was assessed through cell culture and intragastrically administering to mice. The anti-biofilm and anti-caries effects were investigated invivo. The inhibitive mechanism was detected by isobaric tag for relative and absolute quantification (itraq) and RT-QPCR. In vitro and invivo study revealed that 2-AI/T significantly inhibited biofilm formation of Strep.mutans and is more so than inhibiting planktonic cells without toxicity. The ribosome and histidine metabolism pathways of Strep.mutans were significantly regulated by this compound. ConclusionsThese results suggest that the 2-AI/T conjugate is a potent inhibitor that can be potentially developed into a new drug to treat and prevent dental caries. Significance and Impact of the StudyThis is the first study to use small molecule from marine natural products, to protect from dental caries invivo. It has potential broad range application in clinical caries prevention, or as a bioactive ingredient for food applications.