Electrochemiluminescence Arrays for Studies of Metabolite-related Toxicity

被引：5

作者：

Bano, Kiran ^{[1
]}

Rusling, James F. ^{[1
,2
,3
,4
,5
]}

机构：

[1] Univ Connecticut, Dept Chem, Storrs, CT 06269 USA

[2] Univ Connecticut, Ctr Hlth, Dept Surg, Farmington, CT 06032 USA

[3] Univ Connecticut, Ctr Hlth, Neag Canc Ctr, Farmington, CT 06032 USA

[4] Univ Connecticut, Inst Mat Sci, Storrs, CT 06269 USA

[5] NationalUniv Ireland, Sch Chem, Galway, Ireland

来源：

ELECTROANALYSIS | 2016年 / 28卷 / 11期

关键词：

Metabolites; Toxicity Chemistry; Electrochemiluminescence; DNA Arrays; DNA Damage; Cytochrome P450s; LAYER-BY-LAYER; DNA-DAMAGE; CYTOCHROME-P450; ENZYMES; OXIDATIVE STRESS; ULTRATHIN FILMS; CHEMICAL TOXICITY; SENSOR; ELECTROCHEMISTRY; MYOGLOBIN;

D O I：

10.1002/elan.201600207

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This article reviews recent progress from our laboratory in electrochemiluminescence (ECL) arrays designed for screening toxicity-related chemistry of chemical and drug candidates. Cytochrome P450s and metabolic bioconjugation enzymes convert lipophilic chemicals in our bodies by oxidation and bioconjugation that can lead to toxic metabolites. DNA can be used as an easily measurable toxicity-related endpoint, targeting DNA oxidation and addcut formation with metabolites. ECL using guanosines in the DNA strands as co-reactants have been used in high throughput arrays utilizing DNA-enzyme films fabricated layer-by-layer. This review describes approaches developed to provide new high throughput ECL arrays to aid in toxicity assessment for drug and chemical product development.

引用

页码：2636 / 2643

页数：8

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