Electrochemiluminescence Arrays for Studies of Metabolite-related Toxicity

被引：5
作者：
Bano, Kiran [1 ]
Rusling, James F. [1 ,2 ,3 ,4 ,5 ]
机构：
[1] Univ Connecticut, Dept Chem, Storrs, CT 06269 USA
[2] Univ Connecticut, Ctr Hlth, Dept Surg, Farmington, CT 06032 USA
[3] Univ Connecticut, Ctr Hlth, Neag Canc Ctr, Farmington, CT 06032 USA
[4] Univ Connecticut, Inst Mat Sci, Storrs, CT 06269 USA
[5] NationalUniv Ireland, Sch Chem, Galway, Ireland
来源：
ELECTROANALYSIS | 2016年 / 28卷 / 11期
关键词：
Metabolites; Toxicity Chemistry; Electrochemiluminescence; DNA Arrays; DNA Damage; Cytochrome P450s; LAYER-BY-LAYER; DNA-DAMAGE; CYTOCHROME-P450; ENZYMES; OXIDATIVE STRESS; ULTRATHIN FILMS; CHEMICAL TOXICITY; SENSOR; ELECTROCHEMISTRY; MYOGLOBIN;
D O I：
10.1002/elan.201600207
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
This article reviews recent progress from our laboratory in electrochemiluminescence (ECL) arrays designed for screening toxicity-related chemistry of chemical and drug candidates. Cytochrome P450s and metabolic bioconjugation enzymes convert lipophilic chemicals in our bodies by oxidation and bioconjugation that can lead to toxic metabolites. DNA can be used as an easily measurable toxicity-related endpoint, targeting DNA oxidation and addcut formation with metabolites. ECL using guanosines in the DNA strands as co-reactants have been used in high throughput arrays utilizing DNA-enzyme films fabricated layer-by-layer. This review describes approaches developed to provide new high throughput ECL arrays to aid in toxicity assessment for drug and chemical product development.
引用
收藏
页码：2636 / 2643
页数：8
相关论文
共 41 条
[1]  
Bard A.J., 2004, Electrogenerated Chemiluminescence
[2]   Electrochemiluminescent Array to Detect Oxidative Damage in dsDNA Using [Os(bpy)2(phen-benz-COOH)]2+/Nafion/Graphene Films [J].
Bist, Itti ;
Song, Boya ;
Mosa, Islam M. ;
Keyes, Tia E. ;
Martin, Aaron ;
Forster, Robert J. ;
Rusling, James F. .
ACS SENSORS, 2016, 1 (03) :272-278
[3]   Simultaneous direct electrochemiluminescence and catalytic voltammetry detection of DNA in ultrathin films [J].
Dennany, L ;
Forster, RJ ;
Rusling, JF .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (17) :5213-5218
[4]   Direct electrochemiluminescence detection of oxidized DNA in ultrathin films containing [Os(bpy)2(PVP)10]2+ [J].
Dennany, L ;
Forster, RJ ;
White, B ;
Smyth, M ;
Rusling, JF .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (28) :8835-8841
[5]   Some Current Insights into Oxidative Stress [J].
Durackova, Z. .
PHYSIOLOGICAL RESEARCH, 2010, 59 (04) :459-469
[6]   Recent progress in cytochrome P450 enzyme electrochemistry [J].
Fleming, Barry D. ;
Johnson, Daniel L. ;
Bond, Alan M. ;
Martin, Lisandra L. .
EXPERT OPINION ON DRUG METABOLISM & TOXICOLOGY, 2006, 2 (04) :581-589
[7]   Electrogenerated Chemiluminescence [J].
Forster, Robert J. ;
Bertoncello, Paolo ;
Keyes, Tia E. .
ANNUAL REVIEW OF ANALYTICAL CHEMISTRY, 2009, 2 :359-385
[8]   Molecular Lego: design of molecular assemblies of P450 enzymes for nanobiotechnology [J].
Gilardi, G ;
Meharenna, YT ;
Tsotsou, GE ;
Sadeghi, SJ ;
Fairhead, M ;
Giannini, S .
BIOSENSORS & BIOELECTRONICS, 2002, 17 (1-2) :133-145
[9]   Common and uncommon cytochrome P450 reactions related to metabolism and chemical toxicity [J].
Guengerich, FP .
CHEMICAL RESEARCH IN TOXICOLOGY, 2001, 14 (06) :611-650
[10]   Electrochemiluminescent arrays for cytochrome P450-activated genotoxicity screening.: DNA damage from benzo[a]pyrene metabolites [J].
Hvastkovs, Eli G. ;
So, Minjeong ;
Krishnan, Sadagopan ;
Bajrami, Besnik ;
Tarun, Maricar ;
Jansson, Ingela ;
Schenkman, John B. ;
Rusling, James F. .
ANALYTICAL CHEMISTRY, 2007, 79 (05) :1897-1906
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