Significance of palmitoylation of CD81 on its association with tetraspanin-enriched microdomains and mediating hepatitis C virus cell entry

被引：29

作者：

Zhu, Yong-Zhe ^{[1
]}

Luo, Yuan ^{[1
]}

Cao, Ming-Mei ^{[1
]}

Liu, Yuan ^{[1
]}

Liu, Xiao-Qing ^{[1
]}

Wang, Wen ^{[1
]}

Wu, Da-Ge ^{[1
]}

Guan, Mo ^{[1
]}

Xu, Qing-Qiang ^{[1
]}

Ren, Hao ^{[1
]}

Zhao, Ping ^{[1
]}

Qi, Zhong-Tian ^{[1
]}

机构：

[1] Second Mil Med Univ, Shanghai Key Lab Med Biodef, Dept Microbiol, Shanghai 200433, Peoples R China

来源：

VIROLOGY | 2012年 / 429卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Hepatitis C virus; CD81; Palmitoylation; Tetraspanin-enriched microdomains; Cell entry; MEMBRANE MICRODOMAINS; MAJOR CD9; INFECTION; PROTEINS; CHOLESTEROL; PARTNER; DOMAINS; EWI-2; WEB; ALPHA(3)BETA(1);

D O I：

10.1016/j.virol.2012.03.002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

CD81, a co-receptor for hepatitis C virus (HCV), is a member of the tetraspanin superfamily and is heavily palmitoylated in the juxtamembrane cysteine residues. Palmitoylation plays an important role in protein-protein interactions and association with cholesterol-rich domains of membranes. In this study, Huh7 cells expressing wild-type or palmitoylation-defective CD81 were generated to analyze whether palmitoylation of CD81 is involved in HCV cell entry. Our data showed that de-palmitoylation of CD81 dramatically reduced its association with tetraspanin CD151, but did not influence CD81 partition in detergent-resistant membranes. Moreover, de-palmitoylated CD81 decreased the host cell susceptibility to HCV. Notably, CD151-specific antibodies and siRNA inhibited HCV cell entry, and detachment of CD81 with CD151 decreased the lateral movement of virus particle/CD81 complex to areas of cell-cell contact. These results suggest that palmitoylation of CD81 should facilitate HCV entry, at least in part, by regulating the association of CD81 with tetraspanin-enriched microdomains. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：112 / 123

页数：12

共 58 条

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