ZnS quantum dots as pH probes for study of enzyme reaction kinetics

被引：15

作者：

Wu, Dudu ^{[1
]}

Chen, Zhi ^{[2
]}

机构：

[1] Guangdong Med Coll, Sch Pharm, Dongguan 523808, Peoples R China

[2] Guangdong Med Coll, Ctr Anal, Dongguan 523808, Peoples R China

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2012年 / 51卷 / 01期

关键词：

ZnS quantum dots; Fluorescence intensity; pH probe; Enzyme; Hydrolysis; CDSE NANOCRYSTALS; NANO-ZNS; FLUORESCENCE; PHOTOLUMINESCENCE;

D O I：

10.1016/j.enzmictec.2012.03.012

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Water soluble ZnS quantum clots (QDs) modified by mercaptoacetic acid (MAA) were used to determinate proton concentration in aqueous solutions by fluorescence spectroscopic technique. The results showed that the fluorescence of the water-soluble QDs could be quenched by proton concentration and the fluorescence intensity of the water-soluble QDs decreased linearly as the pH varied from 4.5 to 7.0. Based on this phenomenon, a convenient, rapid and specific method to determine of enzyme reaction kinetics was proposed. The modified ZnS QDs were successfully used as pH probes in monitoring the hydrolysis of glycidyl butyrate catalyzed by porcine pancreatic lipase (PPL). The proposed method was found to improve stability, sensitivity and a monitoring range for determination proton concentration as compared to the already described analytical methods based on p-Nitrophenoxide (PNP). (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：47 / 52

页数：6

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