Offline and Online Liquid Chromatography Mass Spectrometry in Quantitative Proteomics

被引：12

作者：

Bereszczak, Jessica Z. ^{[1
]}

Brancia, Francesco L. ^{[1
]}

机构：

[1] Shimadzu Res Lab Europe, Manchester M17 1GP, Lancs, England

来源：

COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING | 2009年 / 12卷 / 02期

关键词：

LC-MALDI MS; PROTEIN IDENTIFICATION TECHNOLOGY; LASER-DESORPTION/IONIZATION-TIME; DROPLET DEPOSITION INTERFACE; LABELING DERIVATIZATION GLAD; PEPTIDE RETENTION TIME; REVERSED-PHASE HPLC; ION-TRAP; ABSOLUTE QUANTIFICATION; RELATIVE QUANTIFICATION;

D O I：

10.2174/138620709787315418

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Hyphenation with liquid chromatography has become indispensable in mass spectrometry-based proteomics. Sample complexity together with the large variations in dynamic range can be only tackled using techniques that isolate and/or concentrate individual components prior to mass spectrometric analysis. In this review the most recent developments in micro/nanoliquid chromatography interfaced with MALDI and electrospray ionisation are discussed. Particular attention is focused on all applications related to quantitative proteomics.

引用

页码：185 / 193

页数：9

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