Determination of HER2 Amplification Status on Tumour DNA by Digital PCR

被引：28
|
作者：
Garcia-Murillas, Isaac [1 ]
Lambros, Maryou [1 ]
Turner, Nicholas C. [1 ,2 ]
机构：
[1] Inst Canc Res, Breakthrough Breast Canc Res Ctr, London SW3 6JB, England
[2] Royal Marsden Hosp, Breast Unit, London SW3 6JJ, England
来源：
PLOS ONE | 2013年 / 8卷 / 12期
关键词：
POLYMERASE CHAIN-REACTION; BREAST-CANCER; IN-SITU; HYBRIDIZATION; RECEPTOR;
D O I：
10.1371/journal.pone.0083409
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Determination of the presence of HER2 amplification by quantitative PCR has been challenging, in part due to chromosomal instability and identification of a robust a reference region. We assessed the potential of digital PCR for highly accurate assessment of DNA concentration with EFTUD2 as chromosome 17 reference probe. We assessed a HER2: EFTDU2 ratio by digital PCR assay in the microdissected DNA from 18 HER2 amplified and 58 HER2 non-amplified cancers. The HER2: EFTUD2 ratio had high concordance with conventionally defined HER2 status with a sensitivity of 100% (18/18) and a specificity of 98% (57/58). The HER2: EFTUD2 digital PCR assay has potential to accurately assess HER2 amplification status.
引用
收藏
页数：4
相关论文
共 50 条
  • [41] Determination of Her2 Copy Number in Breast Cancer by highly sensitive digital Droplet PCR Method
    Alinger-Scharinger, B.
    Kronberger, C.
    Hitzl, W.
    Reitsamer, R.
    Klassen-Federspiel, F.
    Hager, M.
    Fischer, T.
    Sotlar, K.
    Jaksch-Bogensperger, H.
    GEBURTSHILFE UND FRAUENHEILKUNDE, 2023, 83 (04) : E1 - E1
  • [42] Manual and digital detection of HER2 status of 2721 circulating tumour cells in patients with metastatic breast cancer
    Brouwer, A.
    van de Wiel, M.
    Peeters, B.
    van Dam, P-J
    Vermeulen, P.
    Peeters, M.
    Van Laere, S.
    Peeters, D.
    Dirix, L.
    CANCER RESEARCH, 2016, 76
  • [43] Droplet digital PCR measurement of HER2 in patients with gastric cancer
    H Kinugasa
    K Nouso
    T Tanaka
    K Miyahara
    Y Morimoto
    C Dohi
    T Matsubara
    H Okada
    K Yamamoto
    British Journal of Cancer, 2015, 112 : 1652 - 1655
  • [44] Droplet digital PCR measurement of HER2 in patients with gastric cancer
    Kinugasa, H.
    Nouso, K.
    Tanaka, T.
    Miyahara, K.
    Morimoto, Y.
    Dohi, C.
    Matsubara, T.
    Okada, H.
    Yamamoto, K.
    BRITISH JOURNAL OF CANCER, 2015, 112 (10) : 1652 - 1655
  • [45] Droplet digital PCR measurement of HER2 in patients with gastric cancer
    Kinugasa, Hideaki
    Nouso, Kazuhiro
    Tanaka, Takehiro
    Miyahara, Koji
    Morimoto, Yuki
    Dohi, Chihiro
    Okada, Hiroyuki
    Yamamoto, Kazuhide
    JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY, 2014, 29 : 14 - 14
  • [46] Determination of HER2 status using both serum HER2 levels and circulating tumor cells in patients with recurrent breast cancer whose primary tumor was HER2 negative or of unknown HER2 status
    Tanja Fehm
    Sven Becker
    Silke Duerr-Stoerzer
    Karl Sotlar
    Volkmar Mueller
    Diethelm Wallwiener
    Nancy Lane
    Erich Solomayer
    Jonathan Uhr
    Breast Cancer Research, 9
  • [47] Determination of HER2 status using both serum HER2 levels and circulating tumor cells in patients with recurrent breast cancer whose primary tumor was HER2 negative or of unknown HER2 status
    Fehm, Tanja
    Becker, Sven
    Duerr-Stoerzer, Silke
    Sotlar, Karl
    Mueller, Volkmar
    Wallwiener, Diethelm
    Lane, Nancy
    Solomayer, Erich
    Uhr, Jonathan
    BREAST CANCER RESEARCH, 2007, 9 (05) : R74
  • [48] Quantification of HER2 autoantibodies in the amplification phenomenon of HER2 in breast cancer
    Lauterlein, Jens-Jacob L.
    Petersen, Eva R. B.
    Olsen, Dorte Aa
    Ostergaard, Birthe
    Brandslund, Ivan
    CLINICAL CHEMISTRY AND LABORATORY MEDICINE, 2011, 49 (05) : 877 - 883
  • [49] Determination of the HER2 amplification status by in situ fluorescent hybridization and concordance with immunohistochemistry for breast cancer samples in Colombia
    Plata, Adriana
    Torres, Maria Mercedes
    Lopez, Rocio
    Andrade, Rafael E.
    COLOMBIA MEDICA, 2013, 44 (02): : 108 - 114
  • [50] Correction for chromosome 17 is critical for determination of true Her2 gene amplification status in breast cancer (BC)
    Larsimont, D
    Durbecq, V
    Desmedt, C
    Salgado, R
    Dal Lago, L
    Lespagnard, L
    Verjat, T
    Sotiriou, C
    LABORATORY INVESTIGATION, 2006, 86 : 32A - 32A
12345