Enhanced cellulase production by Trichoderma viride in a rotating fibrous bed bioreactor

被引：50

作者：

Lan, Tian-Qing ^{[1
]}

Wei, Dong ^{[1
,2
]}

Yang, Shang-Tian ^{[1
,2
]}

Liu, Xiaoguang ^{[3
]}

机构：

[1] S China Univ Technol, State Key Lab Pulp & Paper Engn, Sch Light Ind & Food Sci, Guangzhou 510640, Peoples R China

[2] Ohio State Univ, William G Lowrie Dept Chem & Biomol Engn, Columbus, OH 43210 USA

[3] Univ Alabama, Dept Chem & Biol Engn, Tuscaloosa, AL 35487 USA

来源：

BIORESOURCE TECHNOLOGY | 2013年 / 133卷

关键词：

Cellulase; Sugarcane bagasse; Trichoderma viride; Fibrous-bed bioreactor; Proteome; LACTIC-ACID PRODUCTION; SUGARCANE BAGASSE; ENZYMATIC-HYDROLYSIS; SECRETOME ANALYSIS; RHIZOPUS-ORYZAE; PROTEIN; LIGNOCELLULOSE; EXPRESSION; MUTANT;

D O I：

10.1016/j.biortech.2013.01.088

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Filamentous fungi are widely used to produce cellulase, but how the fermentation conditions affect their production is not well known. In this study, cellulase production by Trichoderma viride in submerged fermentations with free cells in a stirred-tank reactor (STR) and immobilized cells in a rotating fibrous-bed bioreactor (RFBB) were investigated. Compared to free-cell fermentation, immobilized-cell fermentation gave 35.5% higher FPase activity and 69.7% higher saccharification yield of sugarcane bagasse (SCB). The secretory proteins in the fermentation broths were analyzed with two-dimensional gel electrophoresis (2-DE) and MALDI-TOF-TOF mass spectrometry, which identified 24 protein spots with differential expression levels. Among them, cellobiohydrolase CBH II and endoglucanase EG II were highly expressed and secreted in the immobilized-cell fermentation, while the free-cell fermentation produced more CBH I and EG IV. These results showed that immobilized-cell fermentation with T. viride in the RFBB was advantageous for cellulase production. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：175 / 182

页数：8

共 50 条

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