Enhanced osteogenic potential of mesenchymal stem cells from cortical bone: a comparative analysis

被引:39
作者
Fernandez-Moure, Joseph S. [1 ,2 ]
Corradetti, Bruna [2 ,3 ]
Chan, Paige [2 ]
Van Eps, Jeffrey L. [1 ,2 ]
Janecek, Trevor [2 ]
Rameshwar, Pranela [5 ]
Weiner, Bradley K. [4 ]
Tasciotti, Ennio [2 ]
机构
[1] Houston Methodist Hosp, Dept Surg, Houston, TX USA
[2] Houston Methodist Res Inst, Dept Nanomed, Houston, TX 77030 USA
[3] Univ Politecn Marche, Dept Life & Environm Sci, I-60131 Ancona, Italy
[4] Houston Methodist Hosp, Dept Orthoped Surg, Houston, TX 77030 USA
[5] Rutgers New Jersey Med Sch, Dept Med, Newark, NJ 07103 USA
来源
STEM CELL RESEARCH & THERAPY | 2015年 / 6卷
关键词
Cortical bone; Mesenchymal stem cells; Multipotent differentiation ability; Osteogenic potential; Hypoxia; Normoxia; UMBILICAL-CORD BLOOD; HUMAN ADIPOSE-TISSUE; MULTI-LINEAGE CELLS; STROMAL CELLS; PROGENITOR CELLS; PHENOTYPIC CHARACTERIZATION; INTERNATIONAL-SOCIETY; TRABECULAR BONE; MARROW; DIFFERENTIATION;
D O I
10.1186/s13287-015-0193-z
中图分类号
Q813 [细胞工程];
学科分类号
摘要
Introduction: Mesenchymal stem cells (MSCs) hold great promise for regenerative therapies in the musculoskeletal system. Although MSCs from bone marrow (BM-MSCs) and adipose tissue (AD-MSCs) have been extensively characterized, there is still debate as to the ideal source of MSCs for tissue-engineering applications in bone repair. Methods: MSCs were isolated from cortical bone fragments (CBF-MSCs) obtained from patients undergoing laminectomy, selected by fluorescence-activated cell sorting analysis, and tested for their potential to undergo mesodermic differentiation. CBF-MSCs were then compared with BM-MSCs and AD-MSCs for their colony-forming unit capability and osteogenic potential in both normoxia and hypoxia. After 2 and 4 weeks in inducing media, differentiation was assessed qualitatively and quantitatively by the evaluation of alkaline phosphatase (ALP) expression and mineral deposition (Von Kossa staining). Transcriptional activity of osteoblastogenesis-associated genes (Alp, RUNX2, Spp1, and Bglap) was also analyzed. Results: The cortical fraction of the bone contains a subset of cells positive for MSC-associated markers and capable of tri-lineage differentiation. The hypoxic conditions were generally more effective in inducing osteogenesis for the three cell lines. However, at 2 and 4 weeks, greater calcium deposition and ALP expression were observed in both hypoxic and normoxic conditions in CBF-MSCs compared with AD-and BM-MSCs. These functional observations were further corroborated by gene expression analysis, which showed a significant upregulation of Bglap, Alp, and Spp1, with a 22.50 (+/- 4.55)-, 46.56 (+/- 7.4)-, 71.46 (+/- 4.16)-fold increase compared with their uninduced counterparts. Conclusions: This novel population of MSCs retains a greater biosynthetic activity in vitro, which was found increased in hypoxic conditions. The present study demonstrates that quantitative differences between MSCs retrieved from bone marrow, adipose, and the cortical portion of the bone with respect to their osteogenic potential exist and suggests the cortical bone as suitable candidate to use for orthopedic tissue engineering and regenerative medicine.
引用
收藏
页数:13
相关论文
共 72 条
[1]   Twenty years of the International Society for Cellular Therapies: the past, present and future of cellular therapy clinical development [J].
Abbott, Stewart ;
Mackay, Geoff ;
Durdy, Matthew ;
Solomon, Susan ;
Zylberberg, Claudia .
CYTOTHERAPY, 2014, 16 (04) :S112-S119
[2]  
Ahmed TAE, 2014, HISTOL HISTOPATHOL, V29, P669, DOI 10.14670/HH-29.669
[3]   Human Stromal (Mesenchymal) Stem Cells from Bone Marrow, Adipose Tissue and Skin Exhibit Differences in Molecular Phenotype and Differentiation Potential [J].
Al-Nbaheen, May ;
Vishnubalaji, Radhakrishnan ;
Ali, Dalia ;
Bouslimi, Amel ;
Al-Jassir, Fawzi ;
Megges, Matthias ;
Prigione, Alessandro ;
Adjaye, James ;
Kassem, Moustapha ;
Aldahmash, Abdullah .
STEM CELL REVIEWS AND REPORTS, 2013, 9 (01) :32-43
[4]  
Amos PJ, 2010, TISSUE ENG PT A, V16, P1595, DOI [10.1089/ten.tea.2009.0616, 10.1089/ten.TEA.2009.0616]
[5]   Mesenchymal stem cells: Revisiting history, concepts, and assays [J].
Bianco, Paolo ;
Robey, Pamela Gehron ;
Simmons, Paul J. .
CELL STEM CELL, 2008, 2 (04) :313-319
[6]   Critical parameters for the isolation of mesenchymal stem cells from umbilical cord blood [J].
Bieback, K ;
Kern, S ;
Klüter, H ;
Eichler, H .
STEM CELLS, 2004, 22 (04) :625-634
[7]  
Casteilla Louis, 2011, World J Stem Cells, V3, P25, DOI 10.4252/wjsc.v3.i4.25
[8]   Umbilical cord and bone marrow mesenchymal stem cell seeding on macroporous calcium phosphate for bone regeneration in rat cranial defects [J].
Chen, Wenchuan ;
Liu, Jun ;
Manuchehrabadi, Navid ;
Weir, Michael D. ;
Zhu, Zhimin ;
Xu, Hockin H. K. .
BIOMATERIALS, 2013, 34 (38) :9917-9925
[9]   Mesenchymal stem cells from amnion and amniotic fluid in the bovine [J].
Corradetti, B. ;
Meucci, A. ;
Bizzaro, D. ;
Cremonesi, F. ;
Consiglio, A. Lange .
REPRODUCTION, 2013, 145 (04) :391-400
[10]   Size-sieved subpopulations of mesenchymal stem cells from intervascular and perivascular equine umbilical cord matrix [J].
Corradetti, B. ;
Lange-Consiglio, A. ;
Barucca, M. ;
Cremonesi, F. ;
Bizzaro, D. .
CELL PROLIFERATION, 2011, 44 (04) :330-342