On the mechanism of recombination hotspot scanning during double-stranded DNA break resection

被引：30

作者：

Carrasco, Carolina ^{[1
]}

Gilhooly, Neville S. ^{[2
]}

Dillingham, Mark S. ^{[2
]}

Moreno-Herrero, Fernando ^{[1
]}

机构：

[1] CSIC, Ctr Nacl Biotecnol, Dept Macromol Struct, E-28049 Madrid, Spain

[2] Univ Bristol, Sch Biochem, Bristol BS8 1TD, Avon, England

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2013年 / 110卷 / 28期

基金：

欧洲研究理事会; 英国生物技术与生命科学研究理事会;

关键词：

protein motor; single molecule biophysics; DNA-end processing; real-time measurements; protein-DNA interactions; TRANSLOCATING RECBCD ENZYME; ADDAB HELICASE-NUCLEASE; CHI-RECOGNITION; HOMOLOGOUS RECOMBINATION; REGULATORY SEQUENCE; DYNAMIC DISORDER; SUPERCOILED DNA; REAL-TIME; MOLECULE; REVEALS;

D O I：

10.1073/pnas.1303035110

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Double-stranded DNA break repair by homologous recombination is initiated by resection of free DNA ends to produce a 3'-ssDNA overhang. In bacteria, this reaction is catalyzed by helicase-nuclease complexes such as AddAB in a manner regulated by specific recombination hotspot sequences called Crossover hotspot instigator (Chi). We have used magnetic tweezers to investigate the dynamics of AddAB translocation and hotspot scanning during double-stranded DNA break resection. AddAB was prone to stochastic pausing due to transient recognition of Chi-like sequences, unveiling an antagonistic relationship between DNA translocation and sequence-specific DNA recognition. Pauses at bona fide Chi sequences were longer, were nonexponentially distributed, and resulted in an altered velocity upon restart of translocation downstream of Chi. We propose a model for the recognition of Chi sequences to explain the origin of pausing during failed and successful hotspot recognition.

引用

页码：E2562 / E2571

页数：10

共 39 条

[31] Bacterial DNA repair: recent insights into the mechanism of RecBCD, AddAB and AdnAB [J].

Wigley, Dale B. .

NATURE REVIEWS MICROBIOLOGY, 2013, 11 (01) :9-13

[32] Probing 3′-ssDNA loop formation in E-coli RecBCD/RecBC-DNA complexes using non-natural DNA:: A model for "Chi" recognition complexes [J].

Wong, C. Jason ;

Rice, Rachel L. ;

Baker, Nathan A. ;

Ju, Tao ;

Lohman, Timothy M. .

JOURNAL OF MOLECULAR BIOLOGY, 2006, 362 (01) :26-43

[33] DNA double-strand break repair: All's well that ends well [J].

Wyman, Claire ;

Kanaar, Roland .

ANNUAL REVIEW OF GENETICS, 2006, 40 :363-383

[34] Alteration of χ recognition by RecBCD reveals a regulated molecular latch and suggests a channel-bypass mechanism for biological control [J].

Yang, Liang ;

Handa, Naofumi ;

Liu, Bian ;

Dillingham, Mark S. ;

Wigley, Dale B. ;

Kowalczykowski, Stephen C. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2012, 109 (23) :8907-8912

[35] Recombination Hotspots and Single-Stranded DNA Binding Proteins Couple DNA Translocation to DNA Unwinding by the AddAB Helicase-Nuclease [J].

Yeeles, Joseph T. P. ;

van Aelst, Kara ;

Dillingham, Mark S. ;

Moreno-Herrero, Fernando .

MOLECULAR CELL, 2011, 42 (06) :806-816

[36] The AddAB helicase-nuclease catalyses rapid and processive DNA unwinding using a single Superfamily 1A motor domain [J].

Yeeles, Joseph T. P. ;

Gwynn, Emma J. ;

Webb, Martin R. ;

Dillingham, Mark S. .

NUCLEIC ACIDS RESEARCH, 2011, 39 (06) :2271-2285

[37] The processing of double-stranded DNA breaks for recombinational repair by helicase-nuclease complexes [J].

Yeeles, Joseph T. P. ;

Dillingham, Mark S. .

DNA REPAIR, 2010, 9 (03) :276-285

[38] DYNAMIC DISORDER - PASSAGE THROUGH A FLUCTUATING BOTTLENECK [J].

ZWANZIG, R .

JOURNAL OF CHEMICAL PHYSICS, 1992, 97 (05) :3587-3589

[39] RATE-PROCESSES WITH DYNAMIC DISORDER [J].

ZWANZIG, R .

ACCOUNTS OF CHEMICAL RESEARCH, 1990, 23 (05) :148-152

← 1 2 3 4 →