A model of the action potential and underlying membrane currents in a rabbit atrial cell

被引:140
作者
Lindblad, DS
Murphey, CR
Clark, JW
Giles, WR
机构
[1] RICE UNIV, DEPT ELECT & COMP ENGN, HOUSTON, TX 77251 USA
[2] UNIV TEXAS, MED BRANCH, DEPT PHYSIOL & BIOPHYS, GALVESTON, TX 77550 USA
[3] UNIV CALGARY, SCH MED, DEPT MED PHYSIOL, CALGARY, AB T2N 4N1, CANADA
[4] UNIV CALGARY, SCH MED, DEPT MED, CALGARY, AB T2N 4N1, CANADA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY | 1996年 / 271卷 / 04期
关键词
atrial myocyte; voltage clamp; Hodgkin-Huxley model;
D O I
10.1152/ajpheart.1996.271.4.H1666
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
We have developed a mathematical model of the rabbit atrial myocyte and have used it in an examination of the ionic basis of the atrial action potential. Available biophysical data have been incorporated into the model to quantify the specific ultrastructural morphology, intracellular ion buffering, and time- and voltage-dependent currents and transport mechanisms of the rabbit atrial cell. When possible, mathematical expressions describing ionic currents identified in rabbit atrium are based on whole cell voltage-clamp data from enzymatically isolated rabbit atrial myocytes. This membrane model is coupled to equations describing Na+, K+, and Ca2+ homeostasis, including the uptake and release of Ca2+ by the sarcoplasmic reticulum and Ca2+ buffering. The resulting formulation can accurately simulate the whole cell voltage-clamp data on which it is based and provides fits to a family of rabbit atrial cell action potentials obtained at 35 degrees C over a range of stimulus rates (0.2-3.0 Hz). The model is utilized to provide a qualitative prediction of the intracellular Ca2+ concentration transient during the action potential and to illustrate the interactions between membrane currents that underlie repolarization in the rabbit atrial myocyte.
引用
收藏
页码:H1666 / H1696
页数:31
相关论文
共 115 条
[1]   RELAXATION IN RABBIT AND RAT CARDIAC-CELLS - SPECIES-DEPENDENT DIFFERENCES IN CELLULAR MECHANISMS [J].
BASSANI, JWM ;
BASSANI, RA ;
BERS, DM .
JOURNAL OF PHYSIOLOGY-LONDON, 1994, 476 (02) :279-293
[2]   CA-2+ CYCLING BETWEEN SARCOPLASMIC-RETICULUM AND MITOCHONDRIA IN RABBIT CARDIAC MYOCYTES [J].
BASSANI, JWM ;
BASSANI, RA ;
BERS, DM .
JOURNAL OF PHYSIOLOGY-LONDON, 1993, 460 :603-621
[3]   2 KINDS OF CALCIUM CHANNELS IN CANINE ATRIAL CELLS - DIFFERENCES IN KINETICS, SELECTIVITY AND PHARMACOLOGY [J].
BEAN, BP .
JOURNAL OF GENERAL PHYSIOLOGY, 1985, 86 (01) :1-30
[4]   RECONSTRUCTION OF ACTION POTENTIAL OF VENTRICULAR MYOCARDIAL FIBERS [J].
BEELER, GW ;
REUTER, H .
JOURNAL OF PHYSIOLOGY-LONDON, 1977, 268 (01) :177-210
[5]  
BERNE RM, 1988, PHYSIOLOGY, P22
[6]   MECHANISM OF RELEASE OF CALCIUM FROM SARCOPLASMIC-RETICULUM OF GUINEA-PIG CARDIAC-CELLS [J].
BEUCKELMANN, DJ ;
WIER, WG .
JOURNAL OF PHYSIOLOGY-LONDON, 1988, 405 :233-255
[7]  
BOYETT MR, 1988, J PHYSIOL-LONDON, V399, P467
[8]   EFFECT OF RATE-DEPENDENT CHANGES IN THE TRANSIENT OUTWARD CURRENT ON THE ACTION-POTENTIAL IN SHEEP PURKINJE-FIBERS [J].
BOYETT, MR .
JOURNAL OF PHYSIOLOGY-LONDON, 1981, 319 (OCT) :23-41
[9]  
BOYETT MR, 1980, PROG BIOPHYS MOL BIO, V36, P1
[10]  
BOYETT MR, 1981, J PHYSIOL-LONDON, V319, P1