EXTRACTION OF LIPASE AND PROTEASE AND CHARACTERIZATION OF ACTIVATED SLUDGE FROM PULP AND PAPER INDUSTRY

被引：13
|
作者：
Karn, Santosh Kr [1 ]
Kumar, Pradeep [1 ]
Pan, Xiangliang [2 ]
机构：
[1] Ambala Coll Engn & Appl Res, Dept Biotechnol Engn, Ambala, India
[2] Chinese Acad Sci, Xianjiang Inst Ecol & Geog, Urumqi 830011, Xinjiang, Peoples R China
来源：
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY | 2013年 / 43卷 / 02期
关键词：
activated sludge; CER; lipase; protease; pulp and paper industry; Triton-X-100; WASTE-WATER; ENZYMES;
D O I：
10.1080/10826068.2012.712589
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Hydrolytic enzymes released by the microorganisms in activated sludge are responsible for the organic matter degradation there; however, the optimal extraction procedure of this valuable resource has not been well established until now. In this study protease and lipase were extracted from activated sludge using ultrasound disintegration combined with a nonionic detergent (Triton X-100) and cation-exchange resin (CER) in combination for the extraction of protease and lipase. It was observed that the concentration of 0.1% and 1% Triton X-100 has a strong influence for the extraction of lipase and protease respectively. Closer study of the enzyme extraction process is essential for different enzymes from activated sludge process.
引用
收藏
页码：152 / 162
页数：11
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