BIOCHEMICAL PROPERTIES OF Bacillus sp ITP-001 LIPASE IMMOBILIZED WITH A SOL GEL PROCESS

被引：15

作者：

Carvalho, Nayara Bezerra ^{[1
]}

Barbosa, Jose Murillo P. ^{[1
]}

Oliveira, Maria Vanessa S. ^{[1
]}

Fricks, Alini T. ^{[1
]}

Lima, Alvaro S. ^{[1
]}

Soares, Cleide Mara F. ^{[1
]}

机构：

[1] Inst Tecnol Pesquisa, BR-49032490 Aracaju, SE, Brazil

来源：

QUIMICA NOVA | 2013年 / 36卷 / 01期

关键词：

biochemical; lipase from Bacillus immobilized; sol gel process; CANDIDA-RUGOSA LIPASE; CATALYTIC-PROPERTIES; THERMOSTABLE LIPASE; HYBRID MATRIX; PURIFICATION; HYDROGEL; ENZYME; SILICA; STRAIN; YEAST;

D O I：

10.1590/S0100-40422013000100010

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

This work presents biochemical characterization of a lipase from a new strain of Bacillus sp. ITP-001, immobilized using a sol gel process (IB). The results from the biochemical characterization of IB showed increased activity for hydrolysis, with 526.63 U g(-1) at pH 5.0 and 80 degrees C, and thermal stability at 37 degrees C. Enzymatic activity was stimulated by ions such as EDTA, Fe+3, Mn+2, Zn+2, and Ca+2, and in various organic solvents. Kinetic parameters obtained for the IB were K-m = 14.62 mM, and V-max = 0.102 mM min(-1) g(-1). The results of biochemical characterization revealed the improved catalytic properties of IB.

引用

页码：52 / 58

页数：7

共 55 条

[1]

Adlercreutz P., 2000, BIOCATALYSIS NONCONV

[2]

[Anonymous], 2003, INPI Patent submission, Patent No. 03068293

[3]

BACHA AB, 2005, ENZYME MICROB TECHNO, V37, P309

[4] BIOCHEMICAL CHARACTERISATION OF LIPASE FROM A NEW STRAIN OF Bacillus sp ITP-001 [J].

Barbosa, Jose Murillo P. ;

Souza, Ranyere L. ;

de Melo, Claudia Moura ;

Fricks, Alini T. ;

Soares, Cleide Mara F. ;

Lima, Alvaro S. .

QUIMICA NOVA, 2012, 35 (06) :1173-1178

[5] Purification of lipase produced by a new source of Bacillus in submerged fermentation using an aqueous two-phase system [J].

Barbosa, Jose Murillo P. ;

Souza, Ranyere L. ;

Fricks, Alini T. ;

Zanin, Gisella Maria ;

Soares, Cleide Mara F. ;

Lima, Alvaro S. .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2011, 879 (32) :3853-3858

[6] Microwave-assisted rapid characterization of lipase selectivities [J].

Bradoo, S ;

Rathi, P ;

Saxena, RK ;

Gupta, R .

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 2002, 51 (02) :115-120

[7] Sequential production of amylolytic and lipolytic enzymes by bacterium strain isolated from petroleum contaminated soil [J].

Carvalho, Nayara Bezerra ;

de Souza, Ranyere Lucena ;

de Castro, Heizir F. ;

Zanin, Gisella M. ;

Lima, Alvaro Silva ;

Soares, Cleide M. F. .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2008, 150 (01) :25-32

[8] Lipase from Pseudomonas aeruginosa LP602: biochemical properties and application for wastewater treatment [J].

Dharmsthiti, S ;

Kuhasuntisuk, B .

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 1998, 21 (1-2) :75-80

[9] A structural perspective on enzymes activated by monovalent cations [J].

Di Cera, E .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (03) :1305-1308

[10] Lipase production by bacterial isolates from petroleum contaminated soil. [J].

Feitosa, Ingrid Cavalcanti ;

de Pinho Barbosa, Jose Murillo ;

Orellana, Sara Cuadros ;

Lima, Alvaro Silva ;

Faria Soares, Cleide Mara .

ACTA SCIENTIARUM-TECHNOLOGY, 2010, 32 (01) :27-31

← 1 2 3 4 5 6 →