A mechanism of resistance to HIV-1 entry: Inefficient interactions of CXCR4 with CD4 and gp120 in macrophages

被引：54

作者：

Dimitrov, DS

Norwood, D

Stantchev, TS

Feng, YR

Xiao, XD

Broder, CC

机构：

[1] NCI, Lab Expt & Computat Biol, DBS, FCRDC,NIH, Frederick, MD 21702 USA

[2] Uniformed Serv Univ Hlth Sci, Dept Microbiol & Immunol, Bethesda, MD 20814 USA

来源：

VIROLOGY | 1999年 / 259卷 / 01期

关键词：

HIV-1; CD4; CXCR4; coreceptors; chemokine receptors; envelope glycoprotein; membrane fusion;

D O I：

10.1006/viro.1999.9747

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

To test the hypothesis that inefficient interactions of CXCR4 with CD4 and gp120 could affect HIV-1 entry, we incubated macrophages, monocytes, and lymphocytes with gp120 and coimmunoprecipitated CD4 by using anti-CXCR4 antibodies. CD4 was efficiently coimmunoprecipitated in lymphocytes and monocytes but not in macrophages. Overexpression of CD4 in macrophages resulted in detection of CD4-CXCR4 and gp120-CD4-CXCR4 complexes in parallel with the restoration of macrophage fusion susceptibility. These results suggest a mechanism of resistance to entry of some X4 HIV-1 strains into macrophages and a method for dissection of the initial stages of HIV entry.

引用

页码：1 / 6

页数：6

共 38 条

[1] A new classification for HIV-1 [J].