Human Polymorphonuclear Neutrophil Responses to Burkholderia pseudomallei in Healthy and Diabetic Subjects

被引：103

作者：

Chanchamroen, Sujin ^{[1
]}

Kewcharoenwong, Chidchamai ^{[1
]}

Susaengrat, Wattanachai ^{[2
]}

Ato, Manabu ^{[3
]}

Lertmemongkolchai, Ganjana ^{[1
]}

机构：

[1] Khon Kaen Univ, Fac Associated Med Sci, Ctr Res & Dev Med Diagnost Labs, Khon Kaen, Thailand

[2] Khon Kaen Hosp, Dept Med, Minist Publ Hlth, Khon Kaen, Thailand

[3] Natl Inst Infect Dis, Dept Immunol, Tokyo, Japan

来源：

INFECTION AND IMMUNITY | 2009年 / 77卷 / 01期

关键词：

COLONY-STIMULATING FACTOR; WHOLE-BLOOD; YERSINIA-ENTEROCOLITICA; PSEUDOMONAS-PSEUDOMALLEI; SPONTANEOUS APOPTOSIS; BACTERIAL PATHOGENS; FLOW-CYTOMETRY; RISK-FACTORS; HOST-CELLS; MELIOIDOSIS;

D O I：

10.1128/IAI.00503-08

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The major predisposing factor for melioidosis is diabetes mellitus, but no immunological mechanisms have been investigated to explain this. In this study, polymorphonuclear neutrophil (PMN) responses to Burkholderia pseudomallei, the causative agent of melioidosis, in healthy and diabetic Thai subjects were determined by flow cytometry. The results showed that B. pseudomallei displayed reduced uptake by PMNs compared to Salmonella enterica serovar Typhimurium and Escherichia coli. Additionally, intracellular survival of B. pseudomallei was detected throughout a 24-h period, indicating the intrinsic resistance of B. pseudomallei to killing by PMNs. Moreover, PMNs from diabetic subjects displayed impaired phagocytosis of B. pseudomallei, reduced migration in response to interleukin-8, and an inability to delay apoptosis. These data show that B. pseudomallei is intrinsically resistant to phagocytosis and killing by PMNs. These observations, together with the impaired migration and apoptosis in diabetes mellitus, may explain host susceptibility in melioidosis.

引用

页码：456 / 463

页数：8

共 45 条

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