Independent regulation of MucD, an HtrA-like protease in Pseudomonas aeruginosa, and the role of its proteolytic motif in alginate gene regulation

被引：43

作者：

Wood, LF

Ohman, DE

机构：

[1] Virginia Commonwealth Univ, Dept Microbiol & Immunol, Richmond, VA 23298 USA

[2] McGuire Dept Vet Affairs Med Ctr, Richmond, VA 23249 USA

来源：

JOURNAL OF BACTERIOLOGY | 2006年 / 188卷 / 08期

关键词：

D O I：

10.1128/JB.188.8.3134-3137.2006

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Expression of mucD, encoding a homologue of the HtrA(DegP) family of endoserine proteases, was investigated in Pseudomonas aeruginosa. Expressed from the algT-mucABCD operon, MucD was detected in mucoid (FRD1) and nonmucoid (PAO1) parental strains and also when polar insertions were placed upstream in algT or mucB. A transcriptional start site for a mucD promoter (PmucD) was mapped within mucC. Expression of single-copy mucD217, encoding MucD altered in the protease motif (S217A), was defective in temperature resistance and alginate gene regulation.

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页码：3134 / 3137

页数：4

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