Comparisons of protocols for decontamination of environmental ice samples for biological and molecular examinations

被引：49
作者：
Rogers, SO
Theraisnathan, V
Ma, LJ
Zhao, Y
Zhang, G
Shin, SG
Castello, JD
Starmer, WT
机构：
[1] Bowling Green State Univ, Dept Biol Sci, Bowling Green, OH 43403 USA
[2] SUNY Coll Environm Sci & Forestry, Fac Environm & Forest Biol, Syracuse, NY 13210 USA
[3] Syracuse Univ, Dept Biol, Syracuse, NY 13244 USA
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2004年 / 70卷 / 04期
关键词：
D O I：
10.1128/AEM.70.4.2540-2544.2004
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Drilling and laboratory manipulations of glacial ice cores introduce contemporary microbes and biomolecules onto the cores. We report herein a systematic comparative study of several decontamination protocols. Only treatment with 5% sodium hypochlorite eliminated all external contaminating microbes and nucleic acids while maintaining the integrity of those within the cores.
引用
收藏
页码：2540 / 2544
页数：5
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