Hydroxyapatite and chitosan isolated and associated with bone marrow in repair of bone tissue in rabbits. Histological and morfometric study

The aim of this research was to evaluate the process of bone regeneration in rabbits by the use of hydroxyapatite and chitosan only and associated with bone marrow. Twenty male and female rabbits, New Zealand breed and weighing in average 3.0 +/- 0.4kg were used. Animals were randomly divided according with two experimental moments, being 10 animals euthanized 30 days after surgery, and 10 euthanized 60 days after surgery. Four implants that constituted the groups were used. Materials were introduced into the proximal (MP) and distal metaphysis (MD) of each femur, so that chitosan was introduced into the MP and hydroxyapatite into the MD of the left limb, and in the right limb bone marrow was added beyond the implants. At the comparative descriptive histological evaluation, it was found that the QUI and QUIMO groups in both periods stimulated more pronounced reactions and accelerated bone repair. In morphometric analysis, QUI and QUIMO groups differed statistically from the other, has been shown greater bone formation (P<0.05).