Antibody cocktail to SARS-CoV-2 spike protein prevents rapid mutational escape seen with individual antibodies

被引：920

作者：

Baum, Alina ^{[1
]}

Fulton, Benjamin O. ^{[1
]}

Wloga, Elzbieta ^{[1
]}

Copin, Richard ^{[1
]}

Pascal, Kristen E. ^{[1
]}

Russo, Vincenzo ^{[1
]}

Giordano, Stephanie ^{[1
]}

Lanza, Kathryn ^{[1
]}

Negron, Nicole ^{[1
]}

Ni, Min ^{[1
]}

Wei, Yi ^{[1
]}

Atwal, Gurinder S. ^{[1
]}

Murphy, Andrew J. ^{[1
]}

Stahl, Neil ^{[1
]}

Yancopoulos, George D. ^{[1
]}

Kyratsous, Christos A. ^{[1
]}

机构：

[1] Regeneron Pharmaceut Inc, Tarrytown, NY 10591 USA

来源：

SCIENCE | 2020年 / 369卷 / 6506期

关键词：

D O I：

10.1126/science.abd0831

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Antibodies targeting the spike protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) present a promising approach to combat the coronavirus disease 2019 (COVID-19) pandemic; however, concerns remain that mutations can yield antibody resistance. We investigated the development of resistance against four antibodies to the spike protein that potently neutralize SARS-CoV-2, individually as well as when combined into cocktails. These antibodies remain effective against spike variants that have arisen in the human population. However, novel spike mutants rapidly appeared after in vitro passaging in the presence of individual antibodies, resulting in loss of neutralization: such escape also occurred with combinations of antibodies binding diverse but overlapping regions of the spike protein. Escape mutants were not generated after treatment with a noncompeting antibody cocktail.

引用

页码：1014 / +

页数：14

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