Positive inductive effect of IL-18 on virus-specific immune responses induced by PRRSV-GP5 DNA vaccine in swine

被引：13

作者：

Zhang, Xiaodong ^{[1
]}

Li, Guojiarig

Gao, Lei ^{[2
]}

Mu, Lianzhi ^{[1
]}

Zhang, Lichun

Cong, Yanlong ^{[1
]}

Ding, Zhuang ^{[1
]}

机构：

[1] Jilin Univ, Coll Anim Sci & Vet Med, Key Lab Zoonosis Res, Minist Educ,Inst Zoonosis, Changchun 130062, Jilin, Peoples R China

[2] Anim Husb & Vet Bur Hebei Prov, Serv Ctr Qianan Anim Husb & Fisheries Technol, Qianan 064400, Hebei, Peoples R China

来源：

RESEARCH IN VETERINARY SCIENCE | 2013年 / 94卷 / 02期

关键词：

PRRSV; GP5; IL-18; Immune response; DNA vaccine; RESPIRATORY SYNDROME VIRUS; CO-EXPRESSING GP5; GENETIC IMMUNIZATION; PASSIVE TRANSFER; M-PROTEINS; PROTECTION; STRAIN; GAMMA; ANTIBODIES; EFFICACY;

D O I：

10.1016/j.rvsc.2012.09.011

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Two DNA vaccines encoding the envelope protein GP5 of porcine reproductive and respiratory syndrome virus (PRRSV) alone (pEGFP-GP5) and co-encoding GP5 and swine interleukin-18 (IL-18) proteins (pEGFP-IL18-GP5), were constructed and comparatively evaluated for their abilities to induce humoral and cellular responses in piglets. Experimental results showed that among all vaccinated groups, the live PRRSV vaccine elicited the highest titre of serum neutralizing antibody and the strongest cell-mediated immune responses, which means that the live PRRSV vaccine is still the first option for the vaccination against PRRSV. Moreover, the piglets inoculated with pEGFP-IL18-GP5 developed significantly higher IFN-gamma production response, significantly increased percentages of CD4+ and CD8+ T-lymphocytes and significantly higher specific T-lymphocyte proliferation response than the pEGFP-GP5 inoculated pigs (P < 0.05). These results demonstrated the positive inductive effect of IL-18 on the activation of cellular immune responses in swines. Therefore, in order to develop a new type vaccine for PRRS prevention and control, co-encoding of GP5 and IL-18 proteins may be a good choice. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：346 / 353

页数：8

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