Use of gel permeation chromatography for clean-up in the analysis of coccidiostats in eggs by liquid chromatography-tandem mass spectrometry

被引：15

作者：

Chico, J. ^{[1
]}

Rubies, A. ^{[2
]}

Centrich, F. ^{[2
]}

Companyo, R. ^{[1
]}

Prat, M. D. ^{[1
]}

Granados, M. ^{[1
]}

机构：

[1] Univ Barcelona, Fac Quim, Dept Quim Analit, E-08028 Barcelona, Spain

[2] Lab Agencia Salut Publ Barcelona, Area Anal Org, Serv Quim, Barcelona 08001, Spain

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2013年 / 405卷 / 14期

关键词：

Food analysis; Coccidiostatics residues; Eggs; GPC; LC-MS-MS; Clean-up; IONOPHORE ANTIBIOTICS; ANIMAL-TISSUES; RESIDUES; SALINOMYCIN; MONENSIN; OIL; FRAGMENTATION; VALIDATION; EXTRACTION; EFFICIENT;

D O I：

10.1007/s00216-013-6896-z

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An analytical method for determination and confirmation of nine coccidiostatics in eggs is reported. Ethyl acetate is used as extraction solvent, with satisfactory results, and simple automated clean-up is based on gel-permeation chromatography (GPC) . The target compounds are then analysed by liquid chromatography-electrospray ionization-tandem mass spectrometry. The method was validated in-house in accordance with Commission Decision 2002/657/EC. Trueness and precision were determined at four concentrations, and the mean errors obtained were < 10 %, with relative standard deviations ranging from 3 to 18 %. For three non-authorized coccidiostatics (clopidol, ethopabate, and ronizadole), decision limit and detection capability were in the ranges 0.12-0.16 and 0.18-0.23 mu g kg(-1), respectively. The results obtained prove the suitability of this new analytical method for routine monitoring of these substances in eggs.

引用

页码：4777 / 4786

页数：10

共 33 条

[1] Analysis of thyreostatic drugs in thyroid samples by liquid chromatography tandem mass spectrometry: Comparison of two sample treatment strategies [J].

Abuin, S. ;

Companyo, R. ;

Centrich, F. ;

Rubies, A. ;

Prat, M. D. .

JOURNAL OF CHROMATOGRAPHY A, 2008, 1207 (1-2) :17-23

[2]

[Anonymous], 2009, OFF J EUR COMMUN L, VL15, P1

[3]

[Anonymous], 2003, 1184342003 ISO

[4]

[Anonymous], 2002, OFF J EUR COMMUN, DOI DOI 10.1017/CBO9781107415324.004

[5]

[Anonymous], 1994, 3640 US EPA

[6]

[Anonymous], 2009, OFF J EUR COMMUN L, VL40, P7

[7] Tandem mass spectrometry in food safety assessment: The determination of phthalates in olive oil [J].

Cavaliere, Brunella ;

Macchione, Barbara ;

Sindona, Giovanni ;

Tagarelli, Antonio .

JOURNAL OF CHROMATOGRAPHY A, 2008, 1205 (1-2) :137-143

[8] Determination of eleven coccidiostats in animal feed by liquid chromatography-tandem mass spectrometry at cross contamination levels [J].

Cronly, Mark ;

Behan, P. ;

Foley, B. ;

Malone, E. ;

Shearan, P. ;

Regan, L. .

ANALYTICA CHIMICA ACTA, 2011, 700 (1-2) :26-33

[9] Multi-residue method for detecting coccidiostats at carry-over level in feed by HPLC-MS/MS [J].

Delahaut, Ph. ;

Pierret, G. ;

Ralet, N. ;

Dubois, M. ;

Gillard, N. .

FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT, 2010, 27 (06) :801-809

[10] Efficient and sensitive detection of residues of nine coccidiostats in egg and muscle by liquid chromatography-electrospray tandem mass spectrometry [J].

Dubois, M ;

Pierret, G ;

Delahaut, P .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2004, 813 (1-2) :181-189

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