Effects of cancer-associated fibroblasts on the migration and invasion abilities of SGC-7901 gastric cancer cells

被引：26
作者：
Hu, Chengyi [1 ]
Wang, Zhengcai [1 ]
Zhai, Lili [2 ]
Yang, Miling [2 ]
Shan, Lihui [2 ]
Chai, Cuicui [2 ]
Liu, Ming [1 ]
Wang, Lifeng [2 ]
机构：
[1] Harbin Med Univ, Dept Pathol, Hosp 4, Harbin 150001, Peoples R China
[2] Shanghai Jiao Tong Univ, Dept Pathol, Xinhua Hosp, Sch Med, Shanghai 200092, Peoples R China
来源：
ONCOLOGY LETTERS | 2013年 / 5卷 / 02期
关键词：
gastric cancer; cancer-associated fibroblasts; SGC-7901; gastric cancer cells; INCREASED EXPRESSION; STROMAL FIBROBLASTS; ACTIVATION PROTEIN; TUMOR-CELLS; CARCINOMA; BREAST; MICROENVIRONMENT;
D O I：
10.3892/ol.2012.1023
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The aim of the current study was to investigate the correlation of cancer-associated fibroblasts (CAFs) with the migration and invasion abilities of gastric cancer cells. Gastric CAFs were grown in primary cultures. The in vitro model of interaction of SGC7901 gastric cancer cells with gastric CAFs was established by the use of Transwell co-culture cells to analyze the influence of CAFs on the migration and invasion abilities of SGC7901 cells. The results revealed that i) human gastric CAFs highly expressed vimentin, fibroblast-activated protein and smooth muscle actin in the in vitro passage culture process; ii) the migration and invasion ability of SGC-7901 cells in the CAF-conditioned medium group (98.67 +/- 13.49, 34.40 +/- 4.63) were significantly higher compared to those of the DMEM group without serum (78.47 +/- 10.59, 26.93 +/- 3.99; P<0.01). The interactions of CAFs and the extracellular matrix with SGC-7901 cells may significantly increase the migration and invasion abilities of SGC-7901 cells.
引用
收藏
页码：609 / 612
页数：4
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