Rapid sensing of antioxidant capacity based on electrochemiluminescence induced by electrochemically generated reactive oxygen species

被引：21

作者：

Nagatani, Naoki ^{[1
]}

Inoue, Yuki ^{[2
]}

Araki, Akiko ^{[2
]}

Ushijima, Hiromi ^{[3
]}

Hattori, Gen ^{[4
]}

Sakurai, Yasuhiro ^{[4
]}

Ogidou, Yutaka ^{[4
]}

Saito, Masato ^{[2
]}

Tamiya, Eiichi ^{[2
]}

机构：

[1] Okayama Univ Sci, Grad Sch Engn, Dept Appl Chem, Kita Ku, 1-1 Ridai Cho, Okayama 7000005, Japan

[2] Osaka Univ, Dept Appl Phys, Grad Sch Engn, 2-1 Yamadaoka, Suita, Osaka 5650871, Japan

[3] Biodevice Technol Ltd, 2-13 Asahidai, Nomi, Ishikawa 9231211, Japan

[4] Designer Foods Co Ltd, Chikusa Ku, 22-8 Chikusa, Nagoya, Aichi 4640858, Japan

来源：

ELECTROCHIMICA ACTA | 2016年 / 222卷

关键词：

electrochemiluminescence antioxidant capacity; screen-printed electrode; ORAC; ESR; ROS; luminol; SCAVENGING CAPACITY; PHENOLIC-COMPOUNDS; IN-VITRO; ASSAY; CHEMILUMINESCENCE; LUMINOL; MECHANISM; NANOPARTICLES; SUPPLEMENTS; PREVENTION;

D O I：

10.1016/j.electacta.2016.11.012

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

A simple and rapid electrochemiluminescence (ECL) method for the measurement of antioxidant capacity using a portable ECL device with a screen-printed electrode is described. The ECL method is based on the luminous reaction of reactive oxygen species (ROS), luminol radicals and antioxidants. ROS are generated by the electrochemical reduction of dissolved oxygen and competitively react with the luminol and antioxidants. The ECL intensity depends on the antioxidant capacity because the radicals are neutralized by the antioxidants, suppressing the luminous reaction. An antioxidant standard curve was generated using different concentrations of known amounts of Trolox by ECL measurement and the detection limit for antioxidant capacity was found to be 0.06 mM of Trolox. An antioxidant capacity of beverages (22 types) was evaluated by comparing with the standard curve of Trolox. The time necessary for the ECL measurement of antioxidant capacity is only two minutes after combining the sample solution with luminol on a screen-printed electrode. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：580 / 586

页数：7

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