In vitro evaluation of canine hemostasis following dilution with hydroxyethyl starch (130/0.4) via thromboelastometry

被引：42

作者：

Falco, Sara ^{[1
]}

Bruno, Barbara ^{[1
]}

Maurella, Cristiana ^{[2
]}

Bellino, Claudio ^{[1
]}

D'Angelo, Antonio ^{[1
]}

Gianella, Paola ^{[1
]}

Tarducci, Alberto ^{[1
]}

Zanatta, Renato ^{[1
]}

Borrelli, Antonio ^{[1
]}

机构：

[1] Dept Anim Pathol, Clin Med Sect, I-10095 Grugliasco, TO, Italy

[2] Ist Zooprofilatt Sperimentale Piemonte, I-10154 Turin, Italy

来源：

JOURNAL OF VETERINARY EMERGENCY AND CRITICAL CARE | 2012年 / 22卷 / 06期

关键词：

coagulation; colloid; viscoelastic techniques; ROTEM; TEM; BLOOD-COAGULATION; RINGERS SOLUTION; WHOLE-BLOOD; DOGS; HETASTARCH; 6-PERCENT; INFUSION; HYPERCOAGULABILITY; SUBSTITUTION; DEXTRAN-70;

D O I：

10.1111/j.1476-4431.2012.00816.x

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Objective To assess the effects of in vitro hemodilution of canine blood with a low molecular weight hy-droxyethyl starch (HES 130/0.4) by means of thromboelastometry (TEM). Design In vitro experimental study. Setting University Teaching Hospital. Animals Ten healthy adult staff-owned dogs were sampled for the purposes of the study. Samples were also collected from 45 clinically normal dogs to establish thromboelastometric reference intervals. Interventions For each dog whole blood was collected by jugular venipuncture and placed into tubes containing 3.8% trisodium citrate and stored at 37 degrees C. Two standard dilutions (1:4 and 1:10) from canine blood specimens were prepared with HES 130/0.4 and saline 0.9%. Measurements and Main Results The effects of dilution were studied by TEM. No statistically significant differences were observed between the TEM values of the control samples and the samples diluted with saline solution (ratio 1:10 and 1:4). In contrast, hypocoagulability was observed in the samples treated with HES 130/0.4, with more severe effects at 1:4 dilution than at 1:10 dilution. The 1:4 dilution with HES 130/0.4 produced marked alterations: CT (P = 0.035) and CFT (P = 0.0007) on the ex-TEM profile, CT (P = 0.0005) and ML (P = 0.0017) on the fib-TEM profile and CFT (P = 0.0043) on the in-TEM, were all significantly increased (P < 0.05), whereas MCF (P = 0.0042) and alpha angle (P = 0.002) on the in-TEM and MCF (P = 0.011) and alpha angle (P = 0.001) ex-TEM profiles and MCF (P = 0.0001) on the fib-TEM profile were significantly decreased (P < 0.05). Conclusions Dilution of canine blood with HES 130/0.4 results in a thromboelastometric pattern consistent with hypocoagulation and this effect appears to result from a dose-dependent alteration in fibrinogen concentration and inhibition of platelet function. As this is an in vitro study, further in vivo investigations are necessary to confirm the results.

引用

页码：640 / 645

页数：6

共 40 条

[21] In vitro evaluation of the effect of profound haemodilution with hydroxyethyl starch 6%, modified fluid gelatin 4% and dextran 40 10% on coagulation profile measured by thromboelastography [J].

Mortier, E ;

Ongenae, M ;

DeBaerdemaeker, L ;

Herregods, L ;

DenBlauwen, N ;

VanAken, J ;

Rolly, G .

ANAESTHESIA, 1997, 52 (11) :1061-1064

[22] Repetitive large-dose infusion of the novel hydroxyethyl starch 130/0.4 in patients with severe head injury [J].

Neff, TA ;

Doelberg, M ;

Jungheinrich, C ;

Sauerland, A ;

Spahn, DR ;

Stocker, R .

ANESTHESIA AND ANALGESIA, 2003, 96 (05) :1453-1459

[23] Evidence of hypercoagulability in dogs with parvoviral enteritis [J].

Otto, CM ;

Rieser, TM ;

Brooks, MB ;

Russell, MW .

JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION, 2000, 217 (10) :1500-1504

[24] Citrated blood does not reliably reflect fresh whole blood coagulability in trials of in vitro hemodilution [J].

Roche, AM ;

James, MFM ;

Grocott, MPW ;

Mythen, MG .

ANESTHESIA AND ANALGESIA, 2003, 96 (01) :58-61

[25] Hydroxyethylstarch and gelatin solutions impair blood coagulation after cardiac surgery: a prospective randomized trial [J].

Schramko, A. ;

Suojaranta-Ylinen, R. ;

Kuitunen, A. ;

Raivio, P. ;

Kukkonen, S. ;

Niemi, T. .

BRITISH JOURNAL OF ANAESTHESIA, 2010, 104 (06) :691-697

[26] Role of fibrinogen-, factor VIII- and XIII-mediated clot propagation in gelatin haemodilution [J].

Schramko, A. A. ;

Kuitunen, A. H. ;

Suojaranta-Ylinen, R. T. ;

Niemi, T. T. .

ACTA ANAESTHESIOLOGICA SCANDINAVICA, 2009, 53 (06) :731-735

[27] The effect of Hetastarch (670/0.75) in vivo on platelet closure time in the dog [J].

Smart, Lisa ;

Jandrey, Karl E. ;

Kass, Philip H. ;

Wierenga, Janelle R. ;

Tablin, Fern .

JOURNAL OF VETERINARY EMERGENCY AND CRITICAL CARE, 2009, 19 (05) :444-449

[28] THE USE OF HETASTARCH AS ADJUNCT THERAPY IN 26 DOGS WITH HYPOALBUMINEMIA - A PHASE-2 CLINICAL-TRIAL [J].

SMILEY, LE ;

GARVEY, MS .

JOURNAL OF VETERINARY INTERNAL MEDICINE, 1994, 8 (03) :195-202

[29] Clot formation in canine whole blood as measured by rotational thromboelastometry is influenced by sample handling and coagulation activator [J].

Smith, Stephanie A. ;

McMichael, Maureen ;

Galligan, Alyssa ;

Gilor, Shir ;

Hoh, Crystal M. .

BLOOD COAGULATION & FIBRINOLYSIS, 2010, 21 (07) :692-702

[30] HES 130/0.4 impairs haemostasis and stimulates pro-inflammatory blood platelet function [J].

Sossdorf, Maik ;

Marx, Sascha ;

Schaarschmidt, Barbara ;

Otto, Gordon P. ;

Claus, Ralf A. ;

Reinhart, Konrad ;

Hartog, Christiane S. ;

Loesche, Wolfgang .

CRITICAL CARE, 2009, 13 (06) :R208

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