Epigallocatechin-3-gallate enhances key enzymatic activities of hepatic thioredoxin and glutathione systems in selenium-optimal mice but activates hepatic Nrf2 responses in selenium-deficient mice

被引:38
作者
Dong, Ruixia [1 ,3 ]
Wang, Dongxu [1 ,4 ]
Wang, Xiaoxiao [1 ]
Zhang, Ke [1 ]
Chen, Pingping [1 ]
Yang, Chung S. [2 ,4 ]
Zhang, Jinsong [1 ,4 ]
机构
[1] Anhui Agr Univ, State Key Lab Tea Plant Biol & Utilizat, Sch Tea & Food Sci, 130 West Changjiang Rd, Hefei 230036, Anhui, Peoples R China
[2] Rutgers State Univ, Dept Biol Chem, Ernest Mario Sch Pharm, Piscataway, NJ USA
[3] Lishui Vocat & Tech Coll, Dept Forestry & Technol, Lishui, Zhejiang, Peoples R China
[4] Anhui Agr Univ, Int Joint Res Lab Tea Chem & Hlth Effects, Hefei, Anhui, Peoples R China
基金
中国国家自然科学基金;
关键词
Epigallocatechin-3-gallate; Selenium; Thioredoxin system; Glutathione system; Nrf2; response; GREEN TEA POLYPHENOL; ELEMENTAL SELENIUM; REDUCTASE-ACTIVITY; ANTIOXIDANT; CELLS; SELENOPROTEINS; GENES; HEPATOTOXICITY; SULFORAPHANE; PEROXIDASE;
D O I
10.1016/j.redox.2016.10.009
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Selenium participates in the antioxidant defense mainly through a class of selenoproteins, including thioredoxin reductase. Epigallocatechin-3-gallate (EGCG) is the most abundant and biologically active catechin in green tea. Depending upon the dose and biological systems, EGCG may function either as an antioxidant or as an inducer of antioxidant defense via its pro-oxidant action or other unidentified mechanisms. By manipulating the selenium status, the present study investigated the interactions of EGCG with antioxidant defense systems including the thioredoxin system comprising of thioredoxin and thioredoxin reductase, the glutathione system comprising of glutathione and glutathione reductase coupled with glutaredoxin, and the Nrf2 system. In selenium-optimal mice, EGCG increased hepatic activities of thioredoxin reductase, glutathione reductase and glutaredoxin. These effects of EGCG appeared to be not due to overt pro-oxidant action because melatonin, a powerful antioxidant, did not influence the increase. However, in selenium-deficient mice, with low basal levels of thioredoxin reductase 1, the same dose of EGCG did not elevate the above-mentioned enzymes; intriguingly EGCG in turn activated hepatic Nrf2 response, leading to increased heme oxygenase 1 and NAD(P) H:quinone oxidoreductase 1 protein levels and thioredoxin activity. Overall, the present work reveals that EGCG is a robust inducer of the Nrf2 system only in selenium-deficient conditions. Under normal physiological conditions, in selenium-optimal mice, thioredoxin and glutathione systems serve as the first line defense systems against the stress induced by high doses of EGCG, sparing the activation of the Nrf2 system.
引用
收藏
页码:221 / 232
页数:12
相关论文
共 74 条
[1]  
[Anonymous], 2014, COMPUTATIONAL MATH M
[2]  
[Anonymous], J NANOMATER
[3]   Focus on mammalian thioredoxin reductases - Important selenoproteins with versatile functions [J].
Arner, Elias S. J. .
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2009, 1790 (06) :495-526
[4]   TrxR1 and GPx2 are potently induced by isothiocyanates and selenium, and mutually cooperate to protect Caco-2 cells against free radical-mediated cell death [J].
Barrera, Lawrence N. ;
Cassidy, Aedin ;
Wang, Wei ;
Wei, Taotao ;
Belshaw, Nigel J. ;
Johnson, Ian T. ;
Brigelius-Flohe, Regina ;
Bao, Yongping .
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 2012, 1823 (10) :1914-1924
[5]   Epigallocatechin gallate-induced modulation of FoxO signaling in mammalian cells and C. elegans: FoxO stimulation is masked via PI3K/Akt activation by hydrogen peroxide formed in cell culture [J].
Bartholome, Andre ;
Kampkoetter, Andreas ;
Tanner, Stephan ;
Sies, Helmut ;
Klotz, Lars-Oliver .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2010, 501 (01) :58-64
[6]   Glutathione peroxidases [J].
Brigelius-Flohe, Regina ;
Maiorino, Matilde .
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2013, 1830 (05) :3289-3303
[7]   Selenium deficiency activates mouse liver Nrf2-ARE but vitamin E deficiency does not [J].
Burk, Raymond F. ;
Hill, Kristina E. ;
Nakayama, Akihiro ;
Mostert, Volker ;
Levander, Ximena A. ;
Motley, Amy K. ;
Johnson, Delinda A. ;
Johnson, Jeffrey A. ;
Freeman, Michael L. ;
Austin, Lori M. .
FREE RADICAL BIOLOGY AND MEDICINE, 2008, 44 (08) :1617-1623
[8]  
CARLBERG I, 1985, METHOD ENZYMOL, V113, P484
[9]   RETRACTED: Curcumin restores Nrf2 levels and prevents quinolinic acid-induced neurotoxicity (Retracted Article) [J].
Carmona-Ramirez, Ivan ;
Santamaria, Abel ;
Tobon-Velasco, Julio C. ;
Orozco-Ibarra, Marisol ;
Gonzalez-Herrera, Irma G. ;
Pedraza-Chaverri, Jose ;
Maldonado, Perla D. .
JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 2013, 24 (01) :14-24
[10]   S-Glutathionylation of Keap1: a new role for glutathione S-transferase pi in neuronal protection [J].
Carvalho, Andreia Neves ;
Marques, Carla ;
Guedes, Rita C. ;
Castro-Caldas, Margarida ;
Rodrigues, Elsa ;
van Horssen, Jack ;
Gama, Maria Joao .
FEBS LETTERS, 2016, 590 (10) :1455-1466