Role of a peptide tagging system in degradation of proteins synthesized from damaged messenger RNA

被引：886

作者：

Keiler, KC ^{[1
]}

Waller, PRH ^{[1
]}

Sauer, RT ^{[1
]}

机构：

[1] MIT,DEPT BIOL,CAMBRIDGE,MA 02139

来源：

SCIENCE | 1996年 / 271卷 / 5251期

关键词：

D O I：

10.1126/science.271.5251.990

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Variants of lambda repressor and cytochrome b(562) translated from messenger RNAs without stop codons were modified by carboxyl terminal addition of an ssrA-encoded peptide tag and subsequently degraded by carboxyl terminal-specific proteases present in both the cytoplasm and periplasm of Escherichia coli, The tag appears to be added to the carboxyl terminus of the nascent polypeptide chain by cotranslational switching of the ribosome from the damaged messenger RNA to ssrA RNA.

引用

页码：990 / 993

页数：4

共 30 条

[1]

ANTONOV VK, 1993, CHEM PROTEOLYSIS, P36

[2]

BALZI E, 1990, J BIOL CHEM, V265, P7464

[3] Multicopy suppressors of prc mutant Escherichia coli include two HtrA (DegP) protease homologs (HhoAB), DksA, and a truncated RlpA [J].