Absolute Quantification of Uric Acid in Human Urine Using Surface Enhanced Raman Scattering with the Standard Addition Method
被引:133
作者:
Westley, Chloe
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Univ Manchester, Manchester Inst Biotechnol, Sch Chem, 131 Princess St, Manchester M1 7DN, Lancs, EnglandUniv Manchester, Manchester Inst Biotechnol, Sch Chem, 131 Princess St, Manchester M1 7DN, Lancs, England
Westley, Chloe
[1
]
Xu, Yun
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Univ Manchester, Manchester Inst Biotechnol, Sch Chem, 131 Princess St, Manchester M1 7DN, Lancs, EnglandUniv Manchester, Manchester Inst Biotechnol, Sch Chem, 131 Princess St, Manchester M1 7DN, Lancs, England
Xu, Yun
[1
]
Thilaganathan, Baskaran
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St Georges Univ London, London SW17 0RE, England
St Georges Univ Hosp NHS Fdn Trust, Clin Sci Res Ctr, London SW17 0RE, EnglandUniv Manchester, Manchester Inst Biotechnol, Sch Chem, 131 Princess St, Manchester M1 7DN, Lancs, England
Thilaganathan, Baskaran
[2
,3
]
Carnell, Andrew J.
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Univ Liverpool, Dept Chem, Liverpool L69 7ZD, Merseyside, EnglandUniv Manchester, Manchester Inst Biotechnol, Sch Chem, 131 Princess St, Manchester M1 7DN, Lancs, England
Carnell, Andrew J.
[4
]
Turner, Nicholas J.
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Univ Manchester, Manchester Inst Biotechnol, Sch Chem, 131 Princess St, Manchester M1 7DN, Lancs, EnglandUniv Manchester, Manchester Inst Biotechnol, Sch Chem, 131 Princess St, Manchester M1 7DN, Lancs, England
Turner, Nicholas J.
[1
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Goodacre, Royston
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Univ Manchester, Manchester Inst Biotechnol, Sch Chem, 131 Princess St, Manchester M1 7DN, Lancs, EnglandUniv Manchester, Manchester Inst Biotechnol, Sch Chem, 131 Princess St, Manchester M1 7DN, Lancs, England
Goodacre, Royston
[1
]
机构:
[1] Univ Manchester, Manchester Inst Biotechnol, Sch Chem, 131 Princess St, Manchester M1 7DN, Lancs, England
[2] St Georges Univ London, London SW17 0RE, England
[3] St Georges Univ Hosp NHS Fdn Trust, Clin Sci Res Ctr, London SW17 0RE, England
[4] Univ Liverpool, Dept Chem, Liverpool L69 7ZD, Merseyside, England
High levels of uric acid in urine and serum can be indicative of hypertension and the pregnancy related condition, preedampsia. We have developed a simple, cost-effective, portable surface enhanced Raman scattering (SERS) approach for the routine analysis of uric acid at clinically relevant levels in urine patient samples. This approach, combined with the standard addition method (SAM), allows for the absolute quantification of uric acid directly in a complex matrix such as that from human urine. Results are highly comparable and in very good agreement with HPLC results, with an average <9% difference in predictions between the two analytical approaches across all samples analyzed, with SERS demonstrating a 60-fold reduction in acquisition time compared with HPLC. For the first time, clinical prepreedampsia patient samples have been used for quantitative uric acid detection using a simple, rapid colloidal SERS approach without the need for complex data analysis.
机构:
Univ Manchester, Sch Chem, Manchester Inst Biotechnol, Manchester M1 7DN, Lancs, EnglandUniv Manchester, Sch Chem, Manchester Inst Biotechnol, Manchester M1 7DN, Lancs, England
Alharbi, Omar
;
Xu, Yun
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Univ Manchester, Sch Chem, Manchester Inst Biotechnol, Manchester M1 7DN, Lancs, EnglandUniv Manchester, Sch Chem, Manchester Inst Biotechnol, Manchester M1 7DN, Lancs, England
Xu, Yun
;
Goodacre, Royston
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机构:
Univ Manchester, Sch Chem, Manchester Inst Biotechnol, Manchester M1 7DN, Lancs, EnglandUniv Manchester, Sch Chem, Manchester Inst Biotechnol, Manchester M1 7DN, Lancs, England
机构:
Magee Womens Res Inst, Pittsburgh, PA 15213 USA
Univ Pittsburgh, Dept Obstet Gynecol & Reprod Sci, Pittsburgh, PA USAMagee Womens Res Inst, Pittsburgh, PA 15213 USA
Bainbridge, S. A.
;
Roberts, J. M.
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机构:
Magee Womens Res Inst, Pittsburgh, PA 15213 USA
Univ Pittsburgh, Dept Obstet Gynecol & Reprod Sci, Pittsburgh, PA USA
Univ Pittsburgh, Dept Epidemiol, Pittsburgh, PA 15261 USAMagee Womens Res Inst, Pittsburgh, PA 15213 USA
机构:
Univ Manchester, Sch Chem, Manchester Inst Biotechnol, Manchester M1 7DN, Lancs, EnglandUniv Manchester, Sch Chem, Manchester Inst Biotechnol, Manchester M1 7DN, Lancs, England
Alharbi, Omar
;
Xu, Yun
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机构:
Univ Manchester, Sch Chem, Manchester Inst Biotechnol, Manchester M1 7DN, Lancs, EnglandUniv Manchester, Sch Chem, Manchester Inst Biotechnol, Manchester M1 7DN, Lancs, England
Xu, Yun
;
Goodacre, Royston
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机构:
Univ Manchester, Sch Chem, Manchester Inst Biotechnol, Manchester M1 7DN, Lancs, EnglandUniv Manchester, Sch Chem, Manchester Inst Biotechnol, Manchester M1 7DN, Lancs, England
机构:
Magee Womens Res Inst, Pittsburgh, PA 15213 USA
Univ Pittsburgh, Dept Obstet Gynecol & Reprod Sci, Pittsburgh, PA USAMagee Womens Res Inst, Pittsburgh, PA 15213 USA
Bainbridge, S. A.
;
Roberts, J. M.
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机构:
Magee Womens Res Inst, Pittsburgh, PA 15213 USA
Univ Pittsburgh, Dept Obstet Gynecol & Reprod Sci, Pittsburgh, PA USA
Univ Pittsburgh, Dept Epidemiol, Pittsburgh, PA 15261 USAMagee Womens Res Inst, Pittsburgh, PA 15213 USA