Absolute Quantification of Uric Acid in Human Urine Using Surface Enhanced Raman Scattering with the Standard Addition Method

被引:133
作者
Westley, Chloe [1 ]
Xu, Yun [1 ]
Thilaganathan, Baskaran [2 ,3 ]
Carnell, Andrew J. [4 ]
Turner, Nicholas J. [1 ]
Goodacre, Royston [1 ]
机构
[1] Univ Manchester, Manchester Inst Biotechnol, Sch Chem, 131 Princess St, Manchester M1 7DN, Lancs, England
[2] St Georges Univ London, London SW17 0RE, England
[3] St Georges Univ Hosp NHS Fdn Trust, Clin Sci Res Ctr, London SW17 0RE, England
[4] Univ Liverpool, Dept Chem, Liverpool L69 7ZD, Merseyside, England
基金
英国生物技术与生命科学研究理事会;
关键词
QUANTITATIVE DETECTION; SILVER COLLOIDS; CARDIOVASCULAR RISK; SERS; PREECLAMPSIA; SPECTROSCOPY; IDENTIFICATION; BIOMARKERS; SUBSTRATE;
D O I
10.1021/acs.analchem.6b04588
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
High levels of uric acid in urine and serum can be indicative of hypertension and the pregnancy related condition, preedampsia. We have developed a simple, cost-effective, portable surface enhanced Raman scattering (SERS) approach for the routine analysis of uric acid at clinically relevant levels in urine patient samples. This approach, combined with the standard addition method (SAM), allows for the absolute quantification of uric acid directly in a complex matrix such as that from human urine. Results are highly comparable and in very good agreement with HPLC results, with an average <9% difference in predictions between the two analytical approaches across all samples analyzed, with SERS demonstrating a 60-fold reduction in acquisition time compared with HPLC. For the first time, clinical prepreedampsia patient samples have been used for quantitative uric acid detection using a simple, rapid colloidal SERS approach without the need for complex data analysis.
引用
收藏
页码:2472 / 2477
页数:6
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