Response of liver to lipopolysaccharide treatment in male and female rats

被引:20
作者
Depboylu, Bilge [1 ]
Giris, Murat [1 ]
Olgac, Vakur [2 ]
Dogru-Abbasoglu, Semra [1 ]
Uysal, Mujdat [1 ]
机构
[1] Istanbul Univ, Istanbul Fac Med, Dept Biochem, TR-34093 Istanbul, Turkey
[2] Istanbul Univ, Inst Oncol, Dept Pathol, TR-34093 Istanbul, Turkey
关键词
Lipopolysaccharide; Oxidative stress; Necrosis; Apoptosis; Liver; Gender; Rat; MITOCHONDRIAL DYSFUNCTION; GENDER-DIFFERENCES; OXIDATIVE DAMAGE; CELL-DEATH; SEPSIS; APOPTOSIS; MICE; GLUTATHIONE; EXPRESSION; DIMORPHISM;
D O I
10.1016/j.etp.2012.07.004
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Gender is considered to be an important factor in endotoxin-induced tissue damage. Our aim was to examine the role of sex on the prooxidant-antioxidant status, necrotic and apoptotic events in the liver of lipopolysaccharide (LPS)-treated rats. We determined levels of lipid peroxides, non-enzymatic and enzymatic antioxidants, and expressions of apoptosis-related proteins, antiapoptotic B cell lymphoma-2 (Bcl-2) and proapoptotic Bax, caspase-3 activity and apoptotic cell numbers in the liver. Hepatic histopathology and serum alanine transaminase (ALT) and aspartate transaminase (AST) activities were also investigated. Male and female Wistar rats (180-200g) were injected with LPS (10 mg/kg, i.p.) and examinations were performed 6 h after the injection. Significant increases in hepatic thiobarbituric acid reactive substances and diene conjugate levels were observed in male and female rats following LPS treatment. However, there were no changes in hepatic glutathione, vitamin E and vitamin C levels together with superoxide dismutase, glutathione peroxidase and glutathione transferase activities. LPS treatment caused significant increases in serum ALT and AST activities and lymphocyte infiltration and necrotic changes in the livers. Bcl-2 and Bax expressions, caspase-3 activity and apoptotic cell numbers were also found to be increased in both groups. In conclusion, no sex-dependent difference was observed in the changed hepatic prooxidant-antioxidant status of rats following LPS treatment. Besides, the process leading to apoptosis and necrosis in the liver showed a similar pattern in both gender of rats. (C) 2012 Elsevier GmbH. All rights reserved.
引用
收藏
页码:645 / 650
页数:6
相关论文
共 35 条
[1]   Influence of gender on the outcome of severe sepsis - A reappraisal [J].
Adrie, Christophe ;
Azoulay, Elie ;
Francais, Adrien ;
Clec'h, Christophe ;
Darques, Loic ;
Schwebel, Carole ;
Nakache, Didier ;
Jamali, Samir ;
Goldgran-Toledano, Dany ;
Garrouste-Orgeas, Maite ;
Timsit, Jean Francois .
CHEST, 2007, 132 (06) :1786-1793
[2]   Therapeutic effects of erythropoietin in murine models of endotoxin shock [J].
Aoshiba, Kazutetsu ;
Onizawa, Shigemitsu ;
Tsuji, Takao ;
Nagai, Atsushi .
CRITICAL CARE MEDICINE, 2009, 37 (03) :889-898
[3]   The effect of taurine or betaine pretreatment on hepatotoxicity and prooxidant status induced by lipopolysaccharide treatment in the liver of rats [J].
Balkan, J ;
Parildar, FH ;
Dogru-Abbasoglu, S ;
Aykaç-Toker, G ;
Uysal, M .
EUROPEAN JOURNAL OF GASTROENTEROLOGY & HEPATOLOGY, 2005, 17 (09) :917-921
[4]  
BEUTLER E, 1963, J LAB CLIN MED, V61, P882
[5]   Mitochondria from females exhibit higher antioxidant gene expression and lower oxidative damage than males [J].
Borrás, C ;
Sastre, J ;
García-Sala, D ;
Lloret, A ;
Pallardó, FV ;
Viña, J .
FREE RADICAL BIOLOGY AND MEDICINE, 2003, 34 (05) :546-552
[6]  
Buege J A, 1978, Methods Enzymol, V52, P302
[7]   Beneficial pleiotropic actions of melatonin in an experimental model of septic shock in mice:: regulation of pro-/anti-inflammatory cytokine network, protection against oxidative damage and anti-apoptotic effects [J].
Carrillo-Vico, A ;
Lardone, PJ ;
Naji, L ;
Fernández-Santos, JM ;
Martín-Lacave, I ;
Guerrero, JM ;
Calvo, JR .
JOURNAL OF PINEAL RESEARCH, 2005, 39 (04) :400-408
[8]   Skeletal muscle and liver oxidative metabolism in response to a voluntary isocaloric intake of a high fat diet in male and female rats [J].
Catala-Niell, Antoni ;
Estrany, Maria Elena ;
Proenza, Ana Maria ;
Gianotti, Magdalena ;
Llado, Isabel .
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 2008, 22 (1-4) :327-336
[9]  
DESAI DI, 1984, METHOD ENZYMOL, V105, P138, DOI DOI 10.1016/S0076-6879(84)05019-9
[10]   Effects of gender on the severity of sepsis [J].
Erikoglu, M ;
Sahin, M ;
Özer, S ;
Avunduk, MC .
SURGERY TODAY, 2005, 35 (06) :467-472