Down-regulation of miR-106b suppresses the growth of human glioma cells

被引：30

作者：

Zhang, Anling ^{[1
]}

Hao, Jianwei ^{[1
]}

Wang, Kun ^{[1
]}

Huang, Qiang ^{[1
]}

Yu, Kai ^{[1
]}

Kang, Chunsheng ^{[1
]}

Wang, Guangxiu ^{[1
]}

Jia, Zhifan ^{[1
]}

Han, Lei ^{[1
]}

Pu, Peiyu ^{[1
]}

机构：

[1] Tianjin Med Univ Gen Hosp, Key Lab Posttrauma Neurorepair & Regenerat Cent N, Tianjin Key Lab Injuries Variat & Regenerat Nervo, Dept Neurosurg,Minist Educ,Tianjin Neurol Inst, Tianjin 300052, Peoples R China

来源：

JOURNAL OF NEURO-ONCOLOGY | 2013年 / 112卷 / 02期

关键词：

Glioma; miR-106b Expression; miR-106b ASON; RBL2; TUMOR-SUPPRESSOR; BRAIN-TUMORS; MICRORNAS; GLIOBLASTOMA; EXPRESSION; FAMILY; CANCER; GENE; SITES; E2F1;

D O I：

10.1007/s11060-013-1061-2

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Recently, many studies have found that the miR-106b 25 cluster plays an oncogenic role in tumor progression. However, the precise role of each microRNAs (miRNAs) in the cluster is not yet clear. In the present study, we examined the expression of miR-106b in glioma samples and a tissue microarray by real-time PCR and in situ hybridization (ISH), respectively, finding that miR-106b is overexpressed in the majority of gliomas. Meanwhile, the expression of miR-106b was positively correlated with tumor grade (p < 0.05). The transfection of a miR-106b anti-sense oligonucleotide (ASON) into three human glioma cell lines (U251, LN229 and TJ905) suppressed the proliferation of these cells. Moreover, the growth of xenograft tumors in nude mice treated with miR-106b ASON was significantly impaired. A bioinformatics analysis predicted that RBL2 may be the target of miR-106b, and dual-luciferase reporter assays identified RBL2, but not RB1 or RBL1, as a target of miR-106b. These results suggest that miR-106b facilitates glioma cell growth by promoting cell cycle progression through the negative regulation of RBL2.

引用

页码：179 / 189

页数：11

共 31 条

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