Overexpression of MTA3 Correlates with Tumor Progression in Non-Small Cell Lung Cancer

被引：24
作者：
Li, Haiying
Sun, Liangliang
Xu, Ying
Li, Zixuan
Luo, Wenting
Tang, Zhongping
Qiu, Xueshan [1 ]
Wang, Enhua
机构：
[1] China Med Univ, Dept Pathol, Affiliated Hosp 1, Shenyang, Liaoning, Peoples R China
来源：
PLOS ONE | 2013年 / 8卷 / 06期
基金：
中国国家自然科学基金;
关键词：
METASTASIS-ASSOCIATED GENE; CYCLIN D1; COMPLEX; PROTEINS; NURD;
D O I：
10.1371/journal.pone.0066679
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The objective of the current study was to investigate the expression pattern and clinicopathological significance of MTA3 in patients with non-small cell lung cancer (NSCLC). The expression profile of MTA3 in NSCLC tissues and adjacent noncancerous lung tissues was detected by immunohistochemistry. MTA3 was overexpressed in 62 of 108 (57.4%) human lung cancer samples and correlated with p-TNM stage (p < 0.0001), nodal metastasis (p = 0.0009) and poor prognosis (p < 0.05). In addition, the depletion of MTA3 expression with small interfering RNAs inhibited cell growth and colony formation in the A549 and H157 lung cancer cell lines. Moreover, MTA3 depletion induced cell cycle arrest at the G1/S boundary. Western blotting analysis revealed that the knockdown of MTA3 decreased the protein levels of cyclin A, cyclin D1 and p-Rb. These results indicate that MTA3 plays an important role in NSCLC progression.
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页数：8
相关论文
共 26 条
[1]  
Brüning A, 2010, HISTOL HISTOPATHOL, V25, P1447, DOI 10.14670/HH-25.1447
[2]   The metastasis-associated genes MTA1 and MTA3 are abundantly expressed in human placenta and chorionic carcinoma cells [J].
Bruening, Ansgar ;
Makovitzky, Josef ;
Gingelmaier, Andrea ;
Friese, Klaus ;
Mylonas, Ioannis .
HISTOCHEMISTRY AND CELL BIOLOGY, 2009, 132 (01) :33-38
[3]  
D'Angiolella V, 2004, CELL CYCLE, V3, P132
[4]   The metastasis-associated gene MTA1 is upregulated in advanced ovarian cancer, represses ERβ, and enhances expression of oncogenic cytokine GRO [J].
Dannenmann, Christine ;
Shabani, Naim ;
Friese, Klaus ;
Jeschke, Udo ;
Mylonas, Ioannis ;
Bruening, Ansgar .
CANCER BIOLOGY & THERAPY, 2008, 7 (09) :1462-1469
[5]   Timeline - The pathogenesis of cancer metastasis: the 'seed and soil' hypothesis revisited [J].
Fidler, IJ .
NATURE REVIEWS CANCER, 2003, 3 (06) :453-458
[6]   MTA3 and the Mi-2/NuRD complex regulate cell fate during B lymphocyte differentiation [J].
Fujita, N ;
Jaye, DL ;
Geigerman, C ;
Akyildiz, A ;
Mooney, MR ;
Boss, JM ;
Wade, PA .
CELL, 2004, 119 (01) :75-86
[7]  
Gansler T, 2010, CA-CANCER J CLIN, V60, P1, DOI [10.3322/caac.20073, 10.3322/caac.20049]
[8]   Stable histone deacetylase complexes distinguished by the presence of SANT domain proteins CoREST/kiaa0071 and Mta-L1 [J].
Humphrey, GW ;
Wang, YH ;
Russanova, VR ;
Hirai, T ;
Qin, J ;
Nakatani, Y ;
Howard, BH .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (09) :6817-6824
[9]   Genetic background is an important determinant of metastatic potential [J].
Hunter, K ;
Welch, DR ;
Liu, ET .
NATURE GENETICS, 2003, 34 (01) :23-24
[10]   Cyclin D1 overexpression is an indicator of poor prognosis in resectable non small cell lung cancer [J].
Keum, JS ;
Kong, G ;
Yang, SC ;
Shin, DH ;
Park, SS ;
Lee, JH ;
Lee, JD .
BRITISH JOURNAL OF CANCER, 1999, 81 (01) :127-132
123