Polyphosphates and polyphosphatase activity in the yeast Saccharomyces cerevisiae during overexpression of the DDP1 gene

被引：6

作者：

Trilisenko, L. V. ^{[1
]}

Andreeva, N. A. ^{[1
]}

Eldarov, M. A. ^{[2
]}

Dumina, M. V. ^{[2
]}

Kulakovskaya, T. V. ^{[1
]}

机构：

[1] Russian Acad Sci, Skryabin Inst Biochem & Physiol Microorganisms, Pushchino 142290, Moscow Region, Russia

[2] Russian Acad Sci, Bioengn Ctr, Moscow 117312, Russia

来源：

BIOCHEMISTRY-MOSCOW | 2015年 / 80卷 / 10期

基金：

俄罗斯基础研究基金会;

关键词：

polyphosphate; diphosphoinositol pentakisphosphate; bidiphosphoinositol tetrakisphosphate; polyphosphatase; DDP1; gene; Saccharomyces cerevisiae; INORGANIC POLYPHOSPHATE; ESCHERICHIA-COLI; PPN1; GENES; EXOPOLYPHOSPHATASE; ENDOPOLYPHOSPHATASE; PURIFICATION; ACID; PPX1;

D O I：

10.1134/S0006297915100120

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effects of overexpression of yeast diphosphoinositol polyphosphate phosphohydrolase (DDP1) having endopolyphosphatase activity on inorganic polyphosphate metabolism in Saccharomyces cerevisiae were studied. The endopolyphosphatase activity in the transformed strain significantly increased compared to the parent strain. This activity was observed with polyphosphates of different chain length, being suppressed by 2 mM tripolyphosphate or ATP. The content of acid-soluble and acid-insoluble polyphosphates under DDP1 overexpression decreased by 9 and 28%, respectively. The average chain length of salt-soluble and alkali-soluble fractions did not change in the overexpressing strain, and that of acid-soluble polyphosphate increased under phosphate excess. At the initial stage of polyphosphate recovery after phosphorus starvation, the chain length of the acid-soluble fraction in transformed cells was lower compared to the recipient strain. This observation suggests the complex nature of DDP1 involvement in the regulation of polyphosphate content and chain length in yeasts.

引用

页码：1312 / 1317

页数：6

共 24 条

[1] PURIFICATION AND CHARACTERIZATION OF HIGHLY-ACTIVE AND STABLE POLYPHOSPHATASE FROM SACCHAROMYCES-CEREVISIAE CELL-ENVELOPE [J].

ANDREEVA, NA ;

OKOROKOV, LA .

YEAST, 1993, 9 (02) :127-139

[2] Purification and properties of recombinant exopolyphosphatase PPN1 and effects of its overexpression on polyphosphate in Saccharomyces cerevisiae [J].

Andreeva, Nadeshda ;

Trilisenko, Ludmila ;

Kulakovskaya, Tatiana ;

Dumina, Maria ;

Eldarov, Michail .

JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2015, 119 (01) :52-56

[3] Polyphosphatase PPN1 of Saccharomyces cerevisiae: Switching of Exopolyphosphatase and Endopolyphosphatase Activities [J].

Andreeva, Nadezhda ;

Trilisenko, Ludmila ;

Eldarov, Mikhail ;

Kulakovskaya, Tatiana .

PLOS ONE, 2015, 10 (03)

[4] ASSAY OF PROTEINS IN PRESENCE OF INTERFERING MATERIALS [J].

BENSADOUN, A ;

WEINSTEIN, D .

ANALYTICAL BIOCHEMISTRY, 1976, 70 (01) :241-250

[5] Role of β-hydroxybutyrate, its polymer poly-β-hydroxybutyrate and inorganic polyphosphate in mammalian health and disease [J].

Dedkova, Elena N. ;

Blatter, Lothar A. .

FRONTIERS IN PHYSIOLOGY, 2014, 5

[6] Optimization of ordered plasmid assembly by gap repair in Saccharomyces cerevisiae [J].

Eckert-Boulet, Nadine ;

Pedersen, Mette Louise ;

Krogh, Berit Olsen ;

Lisby, Michael .

YEAST, 2012, 29 (08) :323-334

[7] Polyphosphates and exopolyphosphatase activities in the yeast Saccharomyces cerevisiae under overexpression of homologous and heterologous PPN1 genes [J].

Eldarov, M. A. ;

Baranov, M. V. ;

Dumina, M. V. ;

Shgun, A. A. ;

Andreeva, N. A. ;

Trilisenko, L. V. ;

Kulakovskaya, T. V. ;

Ryasanova, L. P. ;

Kulaev, I. S. .

BIOCHEMISTRY-MOSCOW, 2013, 78 (08) :946-953

[8] A NEW AND CONVENIENT COLORIMETRIC DETERMINATION OF INORGANIC ORTHO-PHOSPHATE AND ITS APPLICATION TO THE ASSAY OF INORGANIC PYROPHOSPHATASE [J].

HEINONEN, JK ;

LAHTI, RJ .

ANALYTICAL BIOCHEMISTRY, 1981, 113 (02) :313-317

[9] GUANOSINE PENTAPHOSPHATE PHOSPHOHYDROLASE OF ESCHERICHIA-COLI IS A LONG-CHAIN EXOPOLYPHOSPHATASE [J].

KEASLING, JD ;

BERTSCH, L ;

KORNBERG, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (15) :7029-7033

[10]

Kulaev I S, 2004, BIOCH INORGANIC POLY

← 1 2 3 →