An alternatively spliced transcript of the PHD3 gene retains prolyl hydroxylase activity

被引:30
|
作者
Cervera, AM [1 ]
Apostolova, N [1 ]
Luna-Crespo, F [1 ]
Sanjuan-Pla, A [1 ]
Garcia-Bou, R [1 ]
McCreath, KJ [1 ]
机构
[1] Univ Valencia, Ctr Nacl Invest Cardiovasc, Unidad Mixta Invest, Valencia 46010, Spain
关键词
PHD3; prolyl hydroxylase; hypoxia-inducible factor-1; alternative splicing;
D O I
10.1016/j.canlet.2005.03.004
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Cellular response to limiting oxygen levels is managed, in part, by the transcription factor hypoxia-inducible factor 1 (HIF-1), and the prolyl hydroxylase (PHD) family of oxygen-requiring enzymes. In the process of analyzing the expression of PHD3, we observed the presence of two alternatively processed PHD3 transcripts, designated PHD3 Delta 1 and PHD3 Delta 4. The expression of both PHD3 and PHD3 Delta 1 was observed in all tissues and cell lines tested, although the expression of the novel PHD3 Delta 4 appeared to be restricted to primary cancer tissues. The function of PHD3 Delta 4 was assessed in transfection experiments showing a preserved prolyl hydroxylase activity. We would submit that PHD3 variants generated by alternative splicing may be intrinsically involved in the complex system of oxygen sensing (c) 2005 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:131 / 138
页数:8
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