Identification of two novel membrane proteins from the Tiger frog virus (TFV)

被引：12

作者：

Wang, Qing ^{[1
]}

Luo, Yongwen ^{[1
]}

Xie, Junfeng ^{[1
]}

Dong, Chuanfu ^{[1
]}

Weng, Shaoping ^{[1
]}

Ai, Huashui ^{[1
]}

Lue, Ling ^{[1
]}

Yang, Xiuqun ^{[1
]}

Yu, Xiaoqiang ^{[2
]}

He, Jianguo ^{[1
]}

机构：

[1] Sun Yat Sen Zhongshan Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China

[2] Univ Missouri, Div Cell Biol & Biophys, Sch Biol Sci, Kansas City, KS USA

来源：

VIRUS RESEARCH | 2008年 / 136卷 / 1-2期

基金：

国家高技术研究发展计划(863计划);

关键词：

Tiger frog virus; iridovirus; membrane protein; IFA; immunogold;

D O I：

10.1016/j.virusres.2008.04.013

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The Tiger frog virus (TFV) belongs to the genus Ranavirus in the family Iridoviridae, and its genome was completely sequenced in 2002. In order to better understand the viral structure and functional genes involved in infection and virus-host interactions, two candidate genes, ORF001L and ORF020R, were selected for our study ORF001L and ORF020R were analyzed by genomic comparison and by using the TMHMM software. Both genes were conserved in the genus Ranavirus, may encode putative membrane proteins, and were determined as late genes by temporal analysis. In order to identify whether these two proteins were structural proteins or not, ORF001L and ORF020R were cloned and expressed in the pET32a (+) vector. Antisera against the two proteins were prepared by immunization of mice with purified proteins. Western blot analysis suggested that both ORF001L and ORF020R were structural proteins. Indirect immunofluorescence assay (IFA) revealed that the subcellular location of the two proteins was confined to the cytoplasm, especially at the viral assembly site (AS). Immunogold electron microscopy (IEM) further localized these two proteins, showing that they were envelope proteins.(c) 2008 Elsevier B.V. All rights reserved.

引用

页码：35 / 42

页数：8

共 39 条

[1] Comparison of European systemic piscine and amphibian iridoviruses with Epizootic Haematopoietic Necrosis Virus and Frog Virus 3
Ahne, W
Bearzotti, M
Bremont, M
Essbauer, S
[J]. JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH, 1998, 45 (06): : 373 - 383
[2] ALICIA ES, 2004, SCIENCE, V304, P237
[3] [Anonymous], 1989, Molecular Cloning
[4] Identification and characterization of a novel gene encoding an RGD-containing protein in large yellow croaker iridovirus
Ao, Jingqun
Chen, Xinhua
[J]. VIROLOGY, 2006, 355 (02) : 213 - 222
[5] Temporal and differential gene expression of Singapore grouper iridovirus
Chen, Li Ming
Wang, Fan
Song, Wenjun
Hew, Choy Leong
[J]. JOURNAL OF GENERAL VIROLOGY, 2006, 87 : 2907 - 2915
[6] Cheng SF, 2006, J VIROL METHODS, V135, P173, DOI 10.1016/j.jviromet.2006.03.016
[7] CHINCHAR VG, 2005, FAMILY IRIDOVIRIDAE, P145
[8] Isolation and characterization of a pathogenic iridovirus from cultured grouper (Epinephelus sp.) in Taiwan
Chou, HY
Hsu, CC
Peng, TY
[J]. FISH PATHOLOGY, 1998, 33 (04): : 201 - 206
[9] Genome of invertebrate iridescent virus type 3 (mosquito iridescent virus)
Delhon, Gustavo
Tulman, Edan R.
Afonso, Claudio L.
Lu, Zhiqiang
Becnel, James J.
Moser, Bettina A.
Kutish, Gerald F.
Rock, Daniel L.
[J]. JOURNAL OF VIROLOGY, 2006, 80 (17) : 8439 - 8449
[10] Complete genomic DNA sequence of rock bream iridovirus
Do, JW
Moon, CH
Kim, HJ
Ko, MS
Kim, SB
Son, JH
Kim, JS
An, EJ
Kim, MK
Lee, SK
Han, MS
Cha, SJ
Park, MS
Park, MA
Kim, YC
Kim, JW
Park, JW
[J]. VIROLOGY, 2004, 325 (02) : 351 - 363

← 1 2 3 4 →