Serological and molecular variability of Plum pox virus in transgenic and conventional plums

被引：0

作者：

Zagrai, I. ^{[1
]}

Ravelonandro, M. ^{[1
]}

Scorza, R. ^{[1
]}

Gaboreanu, Ioana ^{[1
]}

Ferencz, Beatrix ^{[1
]}

Popescu, O. ^{[1
]}

Zagrai, Luminita ^{[1
]}

Maxim, A. ^{[1
]}

机构：

[1] Fruit Res Stn Bistrita, 3 Drumul Dumitrei Nou St, Bistrita, Romania

来源：

BULLETIN OF THE UNIVERSITY OF AGRICULTURAL SCIENCE AND VETERINARY MEDICINE, VOL 62, 2005: HORTICULTURE | 2005年 / 62卷

关键词：

Plum pox virus; transgenic and conventional plum; serological and molecular variability;

D O I：

暂无

中图分类号：

F3 [农业经济];

学科分类号：

0202 ; 020205 ; 1203 ;

摘要：

Plum pox or sharka is considered to be the most devastating disease of stone fruit, causing serious yield losses. Using a pathogen-derived resistance strategy as an alternative to conventional breeding, we produced a transgenic plum clone highly resistant to PPV. Environmental safety issues were evaluated on potential hazard related to the release of this virus-resistant transgenic crop. The objective of this study was to examine, under field conditions, the potential risk of a transgenic plum containing the PPV CP (coat protein) gene. These studies focused on the investigation of the diversity of virus populations by comparing the variability of PPV detected in the transgenic trees versus those found in conventional plums. Preliminary analysis based on serological and molecular data revealed that there was no significant difference between PPV isolated from transgenic and conventional plums. Hence, the release of the transgenic plums utilized in this study does not affect the diversity of indigenous PPV populations.

引用

页码：84 / 90

页数：7

共 17 条

[1] CHARACTERISTICS OF MICROPLATE METHOD OF ENZYME-LINKED IMMUNOSORBENT ASSAY FOR DETECTION OF PLANT-VIRUSES [J].

CLARK, MF ;

ADAMS, AN .

JOURNAL OF GENERAL VIROLOGY, 1977, 34 (MAR) :475-483

[2]

Hall T.A., 1999, NUCL ACIDS S SER, V41, P95, DOI DOI 10.1021/BK-1999-0734.CH008

[3]

Kegler H, 1998, ACTA VIROL, V42, P200

[4]

KERLAN C, 1979, ANN PHYTOPATHOL, V11, P241

[5]

MALINOVSKI T, 2005, IN PRESS PLANT DIS

[6]

Nemeth M. V., 1986, Virus, mycoplasma and Rickettsia Diseases of fruit trees

[7] Simultaneous detection and typing of plum pox potyvirus (PPV) isolates by heminested-PCR and PCR-ELISA [J].

Olmos, A ;

Cambra, M ;

Dasi, MA ;

Candresse, T ;

Esteban, O ;

Gorris, MT ;

Asensio, M .

JOURNAL OF VIROLOGICAL METHODS, 1997, 68 (02) :127-137

[8] Resistance of transgenic Prunus domestica to plum pox virus infection [J].

Ravelonandro, M ;

Scorza, R ;

Bachelier, JC ;

Labonne, G ;

Levy, L ;

Damsteegt, V ;

Callahan, AM ;

Dunez, J .

PLANT DISEASE, 1997, 81 (11) :1231-1235

[9] Transgene-based resistance to plum pox virus (Sharka disease) is transferred through interspecific hybridization in Prunus [J].

Ravelonandro, M ;

Briard, P ;

Renaud, R ;

Scorza, R .

PROCEEDINGS OF THE INTERNATIONAL SYMPOSIUM ON MOLECULAR MARKERS FOR CHARACTERIZING GENOTYPES AND IDENTIFYING CULTIVARS IN HORTICULTURE, 2001, (546) :569-574

[10]

RAVELONANDRO M, 2002, PLANTS HLTH MAGAZINE, V6, P16

← 1 2 →