Rapid detection of rifampicin in fish using immunochromatographic strips

被引：7

作者：

Hao, Kai ^{[1
]}

Suryoprabowo, Steven ^{[2
,3
]}

Song, Shanshan ^{[2
,3
]}

Kuang, Hua ^{[2
,3
]}

Liu, Liqiang ^{[2
,3
]}

机构：

[1] Shandong Univ Technol, Sch Agr Engn & Food Sci, Zibo 255049, Shandong, Peoples R China

[2] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi, Jiangsu, Peoples R China

[3] Jiangnan Univ, Sch Food Sci & Technol, Int Joint Res Lab Biointerface & Biodetect, Wuxi, Jiangsu, Peoples R China

来源：

FOOD AND AGRICULTURAL IMMUNOLOGY | 2020年 / 31卷 / 01期

关键词：

Immunochromatographic test; monoclonal antibody; rifampicin; strip test; MONOCLONAL-ANTIBODY; IC-ELISA; ASSAY; METABOLITES; PLASMA; SERUM; HPLC;

D O I：

10.1080/09540105.2020.1753017

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

In this study we developed a rapid and simple assay to detect RFP in fish using an immunochromatographic assay. We produced a sensitive and selective monoclonal antibody against RFP with a half-maximal inhibitory concentration (IC50) of 10.7 ng/mL. Fish samples were extracted with ethyl acetate, then centrifuged at 8000 rpm for 10 min, and concentrated by drying over nitrogen. We found under optimal conditions, that the RFP cut-off limits for the test strip was 10 mu g/mL in both 0.01 M phosphate buffered saline and fish samples, and each test could be completed within 5 min. Therefore, immunochromatographic test can be used as a reliable, rapid, and sensitive method for the detection of RFP in fish.

引用

页码：700 / 710

页数：11

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