Optical sectioning by optical scanning holography and a Wiener filter

I propose a novel digital technique that reduces defocus noise in the reconstruction of the sectional images from the complex hologram of a thick object. In three-dimensional microscopy applications of holography, reducing the defocused light scattered from outside the focused plane is an important issue. In this technique I first extract a complex hologram of a thick object by using optical scanning holography. After that, I separate the power spectra of the focused and defocused planes from the complex hologram. Finally, I construct a Wiener filter by use of the power spectra. The Wiener filter reduces the defocus noise in the reconstruction of the sectional image of the focused plane. Computer simulations show that the proposed Wiener filter reduces the defocus noise and provides the sectional images. (c) 2006 Optical Society of America.